Department of Food Protectants and Infestation Control, CSIR-Central Food Technological Research Institute , Mysore 570020, India.
J Agric Food Chem. 2015 Dec 9;63(48):10518-24. doi: 10.1021/acs.jafc.5b03797. Epub 2015 Nov 20.
The present study was undertaken to investigate the insecticidal activity of chemically characterized Gaultheria procumbens essential oil (EO) and its mode of action against the Coleopteran insects Sitophilus oryzae and Rhyzopertha dominica. Gas chromatography-mass spectrometry results depicted methyl salicylate (MS) as the major compound (96.61%) of EO. EO and its major compound methyl salicylate (MS) showed 100% mortality at 150 and 5.0 μL/L air against S. oryzae and R. dominica, respectively, on 24 h of exposure. The in vivo percent inhibition of AChE activity ranged between 6.12 and 27.50%. In addition, changes in the antioxidative defense system, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and oxidized glutathione (GSSG), in test insects were estimated. A significant dose-dependent response in all test parameters was observed. The results demonstrated that G. procumbens EO could play a significant role in the formulation of EO-based insecticides for the management of stored-grain insects.
本研究旨在探讨化学鉴定的长叶白珠（Gaultheria procumbens）精油（EO）的杀虫活性及其对鞘翅目昆虫米象（Sitophilus oryzae）和玉米象（Rhyzopertha dominica）的作用方式。气相色谱-质谱联用结果表明，EO 的主要化合物为水杨酸甲酯（MS）（96.61%）。EO 及其主要化合物水杨酸甲酯（MS）在暴露 24 小时后，空气中浓度分别为 150 和 5.0 μL/L 时，对米象和玉米象的致死率均达到 100%。AChE 活性的体内抑制率在 6.12%至 27.50%之间。此外，还评估了测试昆虫体内抗氧化防御系统、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）的变化。所有测试参数均表现出显著的剂量依赖性反应。结果表明，长叶白珠精油在基于精油的杀虫剂的配方中可能发挥重要作用，用于防治储粮害虫。
