Jou Li-John, Chen Bo-Ching, Chen Wei-Yu, Liao Chung-Min
Department of Biomechatronic Engineering, National Ilan University, Ilan, Taiwan, 26047, Republic of China.
Department of Post-Modern Agriculture, MingDao University, Changhua, Taiwan, 52345, Republic of China.
Environ Sci Pollut Res Int. 2016 Mar;23(6):5374-89. doi: 10.1007/s11356-015-5735-5. Epub 2015 Nov 13.
This study successfully applied an improved valvometry technique to measure waterborne copper (Cu), based on valve activity dynamics of the freshwater clam Corbicula fluminea. The improved valvometry technique allows the use of free-range bivalves and avoids causing stresses from experimental artifacts. The proposed daily valve rhythm models and a toxicodynamics-based Hill model were linked to predict valve dynamic responses under different Cu exposures with a circadian valve rhythm endpoint. Cu-specific detection threshold was 5.6 (95 % CI 2.1-9.3) and 19.5 (14.6-24.3) μg L(-1) for C. fluminea, based on response times of 300 and 30 min, respectively. Upon exposure to Cu concentrations in excess of 50 μg L(-1), the alteration of valve rhythm behavior was correlated with Cu concentration within 30 min, indicating notable sensing ability. This study outlines the feasibility of an in situ early warning dynamic biomonitoring system for detection of waterborne Cu based on circadian valve activities of C. fluminea.
本研究基于河蚬（Corbicula fluminea）的瓣膜活动动态，成功应用了一种改进的瓣膜测量技术来测定水中的铜（Cu）。改进后的瓣膜测量技术允许使用放养的双壳贝类，避免了实验假象带来的压力。所提出的每日瓣膜节律模型和基于毒理学的希尔模型相联系，以昼夜瓣膜节律终点预测不同铜暴露条件下的瓣膜动态反应。基于300分钟和30分钟的响应时间，河蚬对铜的特定检测阈值分别为5.6（95%置信区间2.1 - 9.3）和19.5（14.6 - 24.3）μg L(-1)。暴露于超过50 μg L(-1)的铜浓度时，瓣膜节律行为的改变在30分钟内与铜浓度相关，表明具有显著的传感能力。本研究概述了基于河蚬昼夜瓣膜活动的原位早期预警动态生物监测系统用于检测水中铜的可行性。
