[2013年宁夏回族自治区手足口病患者分离肠道病毒A10型毒株VP1区基因特征分析]

[Genetic characteristics of VP1 region of coxsackievirus A10 strains isolated from hand foot and mouth disease patients in Ningxia Hui Autonomous Region, 2013].

作者信息

Ma Jiangtao, Yuan Fang, Chen Hui, Ma Xuemin, Zhan Jun, Li Li

机构信息

Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China; Email:

Ningxia Hui Autonomous Region Center for Disease Control and Prevention, Yinchuan 750004, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2015 Jul;36(7):734-7.

PMID:26564704

Abstract

OBJECTIVE

To study the genetic characteristics of VP1 region of coxsackievirus A10 (Cox A10) strains isolated from hand foot and mouth disease (HFMD) cases in Ningxia Hui Autonomous Region (Ningxia) in 2013.

METHODS

A total of 280 specimens, which were identified as non-enterovirus 71 and non-Cox A16 by real-time PCR, were collected and cultured by using RD cell, and the VP1 genes of isolated strains were amplified by using reverse transcriptase PCR (RT-PCR) with degenerated primers and sequenced. The sequencing results were aligned with the sequences in GenBank with BLAST algorithm to identify the virus genotypes. Homologous comparison and phylogenetic analysis were conducted for all the Cox A10 strains identified.

RESULTS

Among 36 virus strains isolated from 280 clinical specimens, 6 were identified as Cox A10. The homologies of nucleotide and amino acid of the Cox A10 strains isolated in Ningxia were 97.0%-99.8% and 99.0%-99.7% respectively, and the Cox A10 strains isolated in Ningxia shared 76.3%-77.2%, 81.6%-83.1%, 94.4%-98.9% and 80.0%-82.3% nucleotide homologies respectively and shared 92.3%-93.0%, 94.0%-95.3%, 98.0%-99.7% and 90.6%-94.0% amino acid homologies respectively with the representative strains of A, B, C and D genotypes. Phylogenetic tree analysis revealed that Cox A10 strains isolated in Ningxia belonged to genotype C.

CONCLUSION

Cox A10 is one of the most common pathogen causing HFMD in Ningxia in 2013. All the Cox A10 stains isolated from HFMD patients in Ningxia belonged to genotype C.

摘要

目的

研究2013年从宁夏回族自治区（宁夏）手足口病（HFMD）病例中分离出的柯萨奇病毒A10（Cox A10）株VP1区的基因特征。

方法

收集280份经实时荧光定量PCR鉴定为非肠道病毒71型和非柯萨奇病毒A16型的标本，采用RD细胞进行培养，用简并引物通过逆转录聚合酶链反应（RT-PCR）扩增分离株的VP1基因并测序。将测序结果用BLAST算法与GenBank中的序列进行比对以鉴定病毒基因型。对所有鉴定出的Cox A10株进行同源性比较和系统进化分析。

结果

从280份临床标本中分离出的36株病毒中，有6株鉴定为Cox A10。宁夏分离的Cox A10株核苷酸和氨基酸同源性分别为97.0% - 99.8%和99.0% - 99.7%，宁夏分离的Cox A10株与A、B、C和D基因型的代表株核苷酸同源性分别为76.3% - 77.2%、81.6% - 83.1%、94.4% - 98.9%和80.0% - 82.3%，氨基酸同源性分别为92.3% - 93.0%、94.0% - 95.3%、98.0% - 99.7%和90.6% - 94.0%。系统进化树分析显示，宁夏分离的Cox A10株属于C基因型。