Tang Chengfang, Li Xiaohong, Wang Feng, Cui Xiaoming, Zhu Yong
Department of Stomatology, Xi'an Medical University, Xi'an 710021, China.
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Zhonghua Kou Qiang Yi Xue Za Zhi. 2015 Jul;50(7):418-22.
To investigate the effects of local application of insulin like growth factor-1 (IGF-1) absorbable gelatin sponge complex in implant fossa on osseointegration around implant in osteoporosis rats.
Female SD rats, aged 4 months, were randomly individed into ovariectomy group (OVX group) and sham-ovariectomy group (sham-ovx group). The rats in OVX group (n = 15) were ovariectomized, and the rats in Sham-OVX group (n = 10) underwent Sham-ovariectomy. Eight weeks later, 5 rats in each group were randomly selected to confirm the osteoporosis model. The ovariectomized rats were randomly divided into osteoporosis group (OP group) and IGF-1 group after the model was successfully established, 5 rats in each group. Pure titanium implants were implanted in the distal part of right femoral epiphyseal in all groups. Absorbable gelatin sponge particles containing 10 µg IGF-1 were placed in the planting fossa in the IGF-1 group, and absorbable gelatin sponge particles without IGF-1 were used in OP group and sham-OVX group. The rats were sacrificed, and then the distal part of right and left femoral epiphyses were taken out to make undecalcified and decalcified tissue sections respectively after 8 weeks. Combined bone lamella width (CBLW) and implant bone contact rate (IBCR) around implant, trabecular width (TW) and trabecular area percentage (TA) around implant and in the cortical bone of left femoral epiphyses were observed by histomorphometric measurement.
The CBLW, IBCR, TW and TA around implant was (55.43 ± 3.50) µm, (81.79 ± 4.45) %, (57.73 ± 4.29) µm and (62.21 ± 7.42) % respectively in sham-OVX group, (60.22 ± 4.70) µm, (83.67 ± 6.63) %, (48.08 ± 3.63) µm and (58.20 ± 8.93) % respectively in IGF-1 group, and (37.11 ± 2.18) µm, (64.60 ± 5.44) %, (41.19 ± 2.93) µm and (42.21 ± 4.34) % respectively in OP group. The CBLW, IBCR and TW around implant had no differences between IGF-1 and sham-OVX group (P > 0.05), which were significantly higher than those in OP group (P < 0.05). The TW and TA of cortical bone in left distal femoral epiphyses was (60.85 ± 6.64) µm, (61.24 ± 6.98) % respectively in sham-OVX group, (38.68 ± 4.74) µm, (43.89 ± 7.76) % respectively IGF-1 group, (40.46 ± 5.38) µm, (44.63 ± 5.39) % respectively in OP group (P < 0.05). The TW and TA of cortical bone in left distal femoral epiphyses had no differences between IGF-1 group and OP group (P > 0.05), which were all significantly lower than those in sham-OVX group (P < 0.05).
The local application of IGF-1 gelatin sponge complex can increase bone tissue around implant and improve osseointegration in osteoporosis.
探讨局部应用胰岛素样生长因子-1（IGF-1）可吸收明胶海绵复合物于种植窝对骨质疏松大鼠种植体周围骨整合的影响。
选取4月龄雌性SD大鼠，随机分为去卵巢组（OVX组）和假去卵巢组（假去势组）。OVX组（n = 15）大鼠行去卵巢手术，假去势组（n = 10）大鼠行假去卵巢手术。8周后，每组随机选取5只大鼠确认骨质疏松模型。模型成功建立后，将去卵巢大鼠随机分为骨质疏松组（OP组）和IGF-1组，每组5只。所有组均于右侧股骨远端骨骺植入纯钛种植体。IGF-1组在种植窝内放置含10 μg IGF-1的可吸收明胶海绵颗粒，OP组和假去势组使用不含IGF-1的可吸收明胶海绵颗粒。8周后处死大鼠，取出左右股骨远端骨骺，分别制作不脱钙和脱钙组织切片。通过组织形态计量学测量观察种植体周围的联合骨板宽度（CBLW）和种植体骨接触率（IBCR）、种植体周围及左侧股骨远端骨骺皮质骨的骨小梁宽度（TW）和骨小梁面积百分比（TA）。
假去势组种植体周围的CBLW、IBCR、TW和TA分别为（55.43 ± 3.50）μm、（81.79 ± 4.45）%、（57.73 ± 4.29）μm和（62.21 ± 7.42）%；IGF-1组分别为（60.22 ± 4.70）μm、（83.67 ± 6.63）%、（48.08 ± 3.63）μm和（58.20 ± 8.93）%；OP组分别为（37.11 ± 2.18）μm、（64.60 ± 5.44）%、（41.19 ± 2.93）μm和（42.21 ± 4.34）%。IGF-1组与假去势组种植体周围的CBLW、IBCR和TW比较，差异无统计学意义（P > 0.05），均显著高于OP组（P < 0.05）。左侧股骨远端骨骺皮质骨的TW和TA，假去势组分别为（60.85 ± 6.64）μm、（61.24 ± 6.98）%；IGF-1组分别为（38.68 ± 4.74）μm、（43.89 ± 7.76）%；OP组分别为（40.46 ± 5.38）μm、（44.63 ± 5.39）%（P < 0.05）。IGF-1组与OP组左侧股骨远端骨骺皮质骨的TW和TA比较，差异无统计学意义（P > 0.05），均显著低于假去势组（P < 0.05）。
局部应用IGF-1明胶海绵复合物可增加骨质疏松大鼠种植体周围的骨组织，改善骨整合。
