Jiang Nan, Du Pinggong, Qu Weidong, Li Lin, Liu Zhonghao, Zhu Songsong
State Key Laboratory of Oral Diseases and Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu; Yantai City Stomatological Hospital, Yantai, People's Republic of China.
Yantai City Stomatological Hospital, Yantai, People's Republic of China.
Int J Nanomedicine. 2016 Sep 16;11:4719-4733. doi: 10.2147/IJN.S113375. eCollection 2016.
For several decades, titanium and its alloys have been commonly utilized for endosseous implantable materials, because of their good mechanical properties, chemical resistance, and biocompatibility. But associated low bone mass, wear and loss characteristics, and high coefficients of friction have limited their long-term stable performance, especially in certain abnormal bone-metabolism conditions, such as postmenopausal osteoporosis. In this study, we investigated the effects of platelet-rich plasma (PRP) treatment and TiO nanoporous modification on the stability of titanium implants in osteoporotic bone. After surface morphology, topographical structure, and chemical changes of implant surface had been detected by scanning electron microscopy (SEM), atomic force microscopy, contact-angle measurement, and X-ray diffraction, we firstly assessed in vivo the effect of PRP treatment on osseointegration of TiO-modified implants in ovariectomized rats by microcomputed tomography examinations, histology, biomechanical testing, and SEM observation. Meanwhile, the potential molecular mechanism involved in peri-implant osseous enhancement was also determined by quantitative real-time polymerase chain reaction. The results showed that this TiO-modified surface was able to lead to improve bone implant contact, while PRP treatment was able to increase the implant surrounding bone mass. The synergistic effect of both was able to enhance the terminal force of implants drastically in biomechanical testing. Compared with surface modification, PRP treatment promoted earlier osteogenesis with increased expression of the and genes and suppressed osteoclastogenesis with increased expression of OPG and decreased levels of RANKL. These promising results show that PRP treatment combined with a TiO-nanomodified surface can improve titanium-implant biomechanical stability in ovariectomized rats, suggesting a beneficial effect to support the success of implants in osteoporotic bone.
几十年来，钛及其合金一直被广泛用作骨内植入材料，因为它们具有良好的机械性能、耐化学性和生物相容性。但与之相关的低骨量、磨损和损耗特性以及高摩擦系数限制了它们的长期稳定性能，尤其是在某些异常骨代谢状况下，如绝经后骨质疏松症。在本研究中，我们调查了富血小板血浆（PRP）处理和TiO纳米多孔改性对骨质疏松骨中钛植入物稳定性的影响。通过扫描电子显微镜（SEM）、原子力显微镜、接触角测量和X射线衍射检测了植入物表面的形态、拓扑结构和化学变化后，我们首先通过微型计算机断层扫描检查、组织学、生物力学测试和SEM观察，在体内评估了PRP处理对去卵巢大鼠中TiO改性植入物骨整合的影响。同时，还通过定量实时聚合酶链反应确定了植入物周围骨增强所涉及的潜在分子机制。结果表明，这种TiO改性表面能够改善骨与植入物的接触，而PRP处理能够增加植入物周围的骨量。两者的协同作用在生物力学测试中能够显著提高植入物的最终受力。与表面改性相比，PRP处理通过增加 和 基因的表达促进了早期成骨，并通过增加骨保护素（OPG）的表达和降低核因子κB受体活化因子配体（RANKL）的水平抑制了破骨细胞生成。这些有前景的结果表明，PRP处理与TiO纳米改性表面相结合可以提高去卵巢大鼠中钛植入物的生物力学稳定性，提示对支持植入物在骨质疏松骨中的成功应用具有有益作用。
