Zhu Hainan, Xie Feng, Luo Xusong, Qin Ling, Sherry Liu X, Scott Levin Lawrence, Li Qingfeng
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA.
Microsurgery. 2016 Nov;36(8):672-675. doi: 10.1002/micr.22530. Epub 2015 Nov 14.
In this report, we present a rat orthotopic forelimb allotransplantation model. Eight forelimbs were transplanted from Brown Norway rats to Lewis rats. Axillary vessels of transplant were used as the vascular pedicles, which were anastomosed to the external jugular vein and common carotid artery of the recipient rat. The ulnar, radial, and median nerves were also repaired. Among rats, a tapered dose of cyclosporine was administered in five rats. In other three rats, no immunosuppressive therapy was given. The viability and signs of rejection of transplanted forelimbs, sensation recovery, bone healing, and histology were assessed up to the 90 postoperative day. All of rats but one survived surgery. All of transplanted forelimbs survived. In the rats treated with cyclosporine the transplanted forelimbs achieved long-term survival with motion and sensation recovery. On 90 day after surgery, bone healing was achieved. There was no sign of rejection in histology. In the rats without cyclosporine treatment, the transplanted forelimbs experienced tissue necrosis started from day 12 postoperatively. This experimental study showed the feasibility of orthotopic forelimb allotransplantation in the rat model. © 2015 Wiley Periodicals, Inc. Microsurgery 36:672-675, 2016.
在本报告中，我们展示了一种大鼠原位前肢同种异体移植模型。将8只前肢从棕色挪威大鼠移植到刘易斯大鼠。移植的腋血管用作血管蒂，将其与受体大鼠的颈外静脉和颈总动脉进行吻合。尺神经、桡神经和正中神经也进行了修复。在大鼠中，5只大鼠给予逐渐减量的环孢素。另外3只大鼠未给予免疫抑制治疗。对移植前肢的存活情况、排斥反应迹象、感觉恢复、骨愈合及组织学进行评估，直至术后90天。除1只大鼠外，所有大鼠均存活至手术结束。所有移植的前肢均存活。在接受环孢素治疗的大鼠中，移植的前肢实现了长期存活，运动和感觉功能得以恢复。术后90天时，实现了骨愈合。组织学检查未发现排斥反应迹象。在未接受环孢素治疗的大鼠中，移植的前肢从术后第12天开始出现组织坏死。本实验研究表明了大鼠原位前肢同种异体移植模型的可行性。© 2015威利期刊公司。显微外科学36:672 - 675，2016。
