Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA.
Microsurgery. 2013 Jan;33(1):51-5. doi: 10.1002/micr.21997. Epub 2012 Jun 18.
Vascularized composite allotransplantation (VCA) has become a clinical reality, prompting research aimed at improving the risk-benefit ratio of such transplants. Here, we report our experience with a gracilis myocutaneous free flap in Massachusetts General Hospital miniature swine as a preclinical VCA model. Fourteen animals underwent free transfer of a gracilis myocutaneous flap comprised of the gracilis muscle and overlying skin, each tissue supplied by independent branches of the femoral vessels. End-to-end anastomoses were performed to the common carotid artery and internal jugular vein, or to the femoral vessels of the recipients. Thirteen of fourteen flaps were successful. A single flap was lost due to compromise of venous outflow. This model allows transplantation of a substantial volume of skin, subcutaneous tissue, and muscle. The anatomy is reliable and easily identified and harvest incurs minimal donor morbidity. We find this gracilis myocutaneous flap an excellent pre-clinical model for the study of vascularized composite allotransplantation.
血管化复合组织移植（VCA）已成为临床现实，促使人们研究旨在提高此类移植的风险效益比。在这里，我们报告了在马萨诸塞州总医院小型猪中使用臀大肌肌皮瓣作为临床前 VCA 模型的经验。14 只动物接受了游离臀大肌肌皮瓣的转移，该皮瓣由臀大肌和覆盖的皮肤组成，每个组织由股血管的独立分支供应。端对端吻合到颈总动脉和颈内静脉，或吻合到受者的股血管。14 个皮瓣中有 13 个成功。一个皮瓣因静脉回流受阻而丢失。该模型允许移植大量的皮肤、皮下组织和肌肉。解剖结构可靠，易于识别，采集过程对供体的发病率极小。我们发现这种臀大肌肌皮瓣是研究血管化复合组织移植的极好的临床前模型。
