Nakayama J, Higuchi R, Kabemura M, Kiryu H, Takenaka A, Hori Y
J Dermatol. 1989 Feb;16(1):31-6. doi: 10.1111/j.1346-8138.1989.tb01215.x.
We have carried out in vitro studies of hyperthermic effects on the viability, blastogenesis, and ultrastructural changes of atypical lymphocytes isolated from the peripheral blood of three patients with malignant lymphoma/leukemia. One was lymphocytic lymphoma/leukemia (T-cell origin), and the others were adult T-cell lymphoma/leukemia (both acute and smoldering type). In the cases of adult T-cell lymphoma/leukemia, the peripheral lymphocytes were found to be more sensitive to hyperthermia than those of non-ATL patients in terms of trypan-blue exclusion ability and blastogenesis ability with concanavalin A challenge. When heated under sublethal conditions, lymphocytes of both ATL and non-ATL patients exhibited the following characteristics: production of multilayered nuclear membranes, increase in vesicles (lysosomes), swelling of mitochondria, and cytoplasmic vacuolization.
我们对三名恶性淋巴瘤/白血病患者外周血中分离出的非典型淋巴细胞进行了热疗对其活力、增殖能力及超微结构变化影响的体外研究。其中一名患者为淋巴细胞性淋巴瘤/白血病（T细胞起源），另外两名是成人T细胞淋巴瘤/白血病（急性型和隐匿型）。在成人T细胞淋巴瘤/白血病患者中，就锥虫蓝排斥能力及伴刀豆球蛋白A激发后的增殖能力而言，外周淋巴细胞对热疗比非成人T细胞淋巴瘤患者的外周淋巴细胞更敏感。在亚致死条件下加热时，成人T细胞淋巴瘤患者和非成人T细胞淋巴瘤患者的淋巴细胞均表现出以下特征：多层核膜形成、囊泡（溶酶体）增多、线粒体肿胀及细胞质空泡化。
