Farhadi Mona, Mohseni Kouchesfahani Homa, Shockravi Abass, Foroozanfar Mosaeeb, Parivar Kazem
Department of Mcrobiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Department of Biology, Kharazmi University, Tehran, Iran.
Iran J Reprod Med. 2015 Aug;13(8):489-94.
Different investigation showed that 5-methoxypsoralen and 8- methoxypsoralen reduce birth rates in the rats.
In this study we worked out the effect of methoxsalen together with ultraviolent A (UVA) radiation on mature Balb/C mice spermatogenesis.
The LD50 standard was determined 160 mg/kg and the UVA dose which causes erythema was calculated 0.046 J/cm2. A sub-lethal dose of 80 mg/kg of methoxsalen solution was injected intrapritoneally to mature mice and after one hour they were exposed to UVA radiation for 20 minutes. Experiments applied included methoxsalen alone, methoxsalen with UVA, UVA alone, sham group (a group received Tween 80), and control group (N=6). In all experimental groups except UVA alone group, injections were carried out, during two consecutive weeks. Serial cross sections (5 µm thickness) were prepared for morphological and histological studies. Tunica albuginea diameter, and number of type A and type B spermatogonia and histological investigation of the testes were measured.
Microscopical and statistical analyses showed significant anomalies among the experimental groups compared to control and sham group. These anomalies included decrease the body weight; increase the relative testis weight; and decrease the number of spermapogonia (type A and B), primary spermatocytes, spermatids and sperms in experimental groups I and II compared to control group. Our results showed the number of spermatozoa in experimental group I was 22.6±2.12, in experimental group II was 33.6±2.05 and in control group was 44.3±2.77 (p<0.05). Moreover in some experimental groups (I and II) shrinkage of seminiferous tubules and release of primary spermatocyte and spermatids were observed to the lumen of them.
It is concluded from the results of this work that treatment with methoxsalen with UVA can damage and disorganize seminiferous tubules and decrease spermatogenic cells.
不同研究表明，5-甲氧基补骨脂素和8-甲氧基补骨脂素可降低大鼠出生率。
本研究旨在探讨甲氧沙林联合紫外线A（UVA）辐射对成熟Balb/C小鼠精子发生的影响。
确定LD50标准为160mg/kg，计算引起红斑的UVA剂量为0.046J/cm²。将80mg/kg的甲氧沙林溶液腹腔注射给成熟小鼠，1小时后对其进行20分钟的UVA辐射。实验包括单独使用甲氧沙林、甲氧沙林联合UVA、单独使用UVA、假手术组（一组接受吐温80）和对照组（N = 6）。除单独使用UVA组外，所有实验组均连续两周进行注射。制备连续横切片（厚度5μm）用于形态学和组织学研究。测量白膜直径、A型和B型精原细胞数量以及睾丸的组织学检查。
显微镜和统计分析显示，与对照组和假手术组相比，实验组存在明显异常。这些异常包括体重减轻；睾丸相对重量增加；实验组I和II中A型和B型精原细胞、初级精母细胞、精子细胞和精子数量与对照组相比减少。我们的结果显示，实验组I的精子数量为22.6±2.12，实验组II为33.6±2.05，对照组为44.3±2.77（p<0.05）。此外，在一些实验组（I和II）中，观察到生精小管萎缩，初级精母细胞和精子细胞释放到管腔中。
从本研究结果可以得出结论，甲氧沙林联合UVA治疗可损伤和破坏生精小管并减少生精细胞。
