• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用改良的RAPD衍生DNA标记对大花栀子进行遗传鉴定

Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers.

作者信息

Mei Zhiqiang, Zhou Boxu, Wei Chunli, Cheng Jingliang, Imani Saber, Chen Hanchun, Fu Junjiang

机构信息

Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China.

State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR) 999078, China.

出版信息

Molecules. 2015 Nov 10;20(11):20219-29. doi: 10.3390/molecules201119687.

DOI:10.3390/molecules201119687
PMID:26569205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6331946/
Abstract

The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random amplified polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides.

摘要

常绿灌木大花栀子（Gardenia jasminoides Ellis var. grandiflora Nakai）是最受欢迎的园林植物之一，具有重要的观赏价值。在此，我们将改良的随机扩增多态性DNA（RAPD）衍生片段克隆到T载体中，并开发了序列特征性扩增区域（SCAR）标记。这些标记已分别以登录号KP641310、KP641311、KP641312和KP641313存入GenBank数据库。数据库的BLAST搜索证实了这些标记的新颖性。四个SCAR标记，即ZZH11、ZZH31、ZZH41和ZZH51，可以特异性识别栀子与其他植物物种的遗传物质。此外，SCAR标记ZZH31可用于区分大花栀子与市场上的其他栀子。总之，本研究通过改良的RAPD衍生DNA标记开发了四个稳定的分子SCAR标记，用于药用和观赏植物栀子的遗传鉴定和认证以及生态保护。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/d25c5e415be1/molecules-20-19687-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/a06ce67af4c7/molecules-20-19687-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/0447b24aec24/molecules-20-19687-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/b6bf635c8e68/molecules-20-19687-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/92969ee2a8a4/molecules-20-19687-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/4e2b0ded164e/molecules-20-19687-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/4401ff9caefb/molecules-20-19687-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/d25c5e415be1/molecules-20-19687-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/a06ce67af4c7/molecules-20-19687-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/0447b24aec24/molecules-20-19687-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/b6bf635c8e68/molecules-20-19687-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/92969ee2a8a4/molecules-20-19687-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/4e2b0ded164e/molecules-20-19687-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/4401ff9caefb/molecules-20-19687-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c384/6331946/d25c5e415be1/molecules-20-19687-g007.jpg

相似文献

1
Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers.利用改良的RAPD衍生DNA标记对大花栀子进行遗传鉴定
Molecules. 2015 Nov 10;20(11):20219-29. doi: 10.3390/molecules201119687.
2
Genetic characterization and authentication of Gardenia jasminoides in different regions of China by using improved RAPD analysis.利用改进的随机扩增多态性DNA分析对中国不同地区栀子的遗传特征及鉴定
Indian J Exp Biol. 2015 Mar;53(3):164-9.
3
Development of SCAR Markers Based on Improved RAPD Amplification Fragments and Molecular Cloning for Authentication of Herbal Medicines Angelica sinensis, Angelica acutiloba and Levisticum officinale.基于改进的随机扩增多态性DNA扩增片段和分子克隆技术开发SCAR标记用于中药材当归、东当归和欧当归的鉴定
Nat Prod Commun. 2015 Oct;10(10):1743-7.
4
Development of RAPD-SCAR markers for different Ganoderma species authentication by improved RAPD amplification and molecular cloning.通过改进随机扩增多态性DNA（RAPD）扩增和分子克隆技术开发用于不同灵芝物种鉴定的RAPD-序列特征扩增区域（SCAR）标记
Genet Mol Res. 2015 May 25;14(2):5667-76. doi: 10.4238/2015.May.25.19.
5
Development and significance of SCAR marker QG12-5 for Canarium album (Lour.) Raeusch by molecular cloning from improved RAPD amplification.基于改良RAPD扩增的分子克隆技术开发榄李（Lour.）Raeusch的SCAR标记QG12-5及其意义
Genet Mol Res. 2016 Aug 26;15(3):gmr8347. doi: 10.4238/gmr.15038347.
6
Development of two novel specific SCAR markers by cloning improved RAPD fragments from the medicinal mushroom Ganoderma lucidium (Leysser: Fr) Karst.通过克隆灵芝（Leysser: Fr）Karst. 中改良的随机扩增多态性DNA（RAPD）片段开发两种新型特异性序列特征扩增区域（SCAR）标记
Genet Mol Res. 2016 Aug 19;15(3):gmr8536. doi: 10.4238/gmr.15038536.
7
Development of RAPD-SCAR markers for Lonicera japonica (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning.通过改进的随机扩增多态性DNA（RAPD）和DNA克隆技术开发用于忍冬（忍冬科）品种鉴定的RAPD-序列特异性扩增区域（SCAR）标记
Rev Biol Trop. 2014 Dec;62(4):1649-57. doi: 10.15517/rbt.v62i4.13493.
8
Identification and determination of geniposide contained in Gardenia jasminoides and in two preparations of mixed traditional Chinese medicines.栀子及两种中药复方制剂中栀子苷的鉴别与含量测定。
J Chromatogr A. 2002 Jun 28;961(1):83-8. doi: 10.1016/s0021-9673(02)00365-5.
9
[Karyological studies on the fruit of Gardenia jasminoides Ellis].[栀子果实的核型研究]
Zhongguo Zhong Yao Za Zhi. 1995 Oct;20(10):588-90, 638.
10
[Molecular ecology of Gardenia jasminoides authenticity].[栀子正品的分子生态学]
Ying Yong Sheng Tai Xue Bao. 2006 Dec;17(12):2385-8.

引用本文的文献

1
Development of an In Vitro Propagation Protocol and a Sequence Characterized Amplified Region (SCAR) Marker of Wall. ex Ging.一种体外繁殖方案的开发以及Wall. ex Ging.的序列特异性扩增区域（SCAR）标记
Plants (Basel). 2020 Feb 14;9(2):246. doi: 10.3390/plants9020246.
2
Development of diagnostic SCAR markers for genomic DNA amplifications in breast carcinoma by DNA cloning of high-GC RAMP-PCR fragments.通过高GC含量的RAMP-PCR片段的DNA克隆开发用于乳腺癌基因组DNA扩增的诊断性SCAR标记。
Oncotarget. 2017 Jul 4;8(27):43866-43877. doi: 10.18632/oncotarget.16704.

本文引用的文献

1
Development of RAPD-SCAR markers for different Ganoderma species authentication by improved RAPD amplification and molecular cloning.通过改进随机扩增多态性DNA（RAPD）扩增和分子克隆技术开发用于不同灵芝物种鉴定的RAPD-序列特征扩增区域（SCAR）标记
Genet Mol Res. 2015 May 25;14(2):5667-76. doi: 10.4238/2015.May.25.19.
2
Screening and identification of potential sex-associated sequences in Danio rerio.斑马鱼中潜在性别相关序列的筛选与鉴定。
Mol Reprod Dev. 2015 Oct;82(10):756-64. doi: 10.1002/mrd.22508. Epub 2015 Jun 16.
3
Genetic characterization and authentication of Gardenia jasminoides in different regions of China by using improved RAPD analysis.
利用改进的随机扩增多态性DNA分析对中国不同地区栀子的遗传特征及鉴定
Indian J Exp Biol. 2015 Mar;53(3):164-9.
4
Comparative assessment of genetic diversity among Indian bamboo genotypes using RAPD and ISSR markers.利用RAPD和ISSR标记对印度竹基因型间的遗传多样性进行比较评估。
Mol Biol Rep. 2015 Aug;42(8):1265-73. doi: 10.1007/s11033-015-3867-9. Epub 2015 Mar 13.
5
Development of RAPD-SCAR markers for Lonicera japonica (Caprifolicaceae) variety authentication by improved RAPD and DNA cloning.通过改进的随机扩增多态性DNA（RAPD）和DNA克隆技术开发用于忍冬（忍冬科）品种鉴定的RAPD-序列特异性扩增区域（SCAR）标记
Rev Biol Trop. 2014 Dec;62(4):1649-57. doi: 10.15517/rbt.v62i4.13493.
6
Anti-inflammatory effects and pharmacokinetics study of geniposide on rats with adjuvant arthritis.栀子苷对佐剂性关节炎大鼠的抗炎作用及药代动力学研究
Int Immunopharmacol. 2015 Jan;24(1):102-9. doi: 10.1016/j.intimp.2014.11.017. Epub 2014 Nov 27.
7
Characterization of four B-chromosome-specific RAPDs and the development of SCAR markers on the maize B-chromosome.玉米B染色体上四个B染色体特异随机扩增多态性DNA的特征分析及序列特异性扩增区域标记的开发
Mol Genet Genomics. 2015 Apr;290(2):431-41. doi: 10.1007/s00438-014-0926-1. Epub 2014 Sep 26.
8
Genetic analysis of litchi (Litchi chinensis Sonn.) in southern China by improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR).利用改良的随机扩增多态性DNA（RAPD）和简单重复序列区间（ISSR）对中国南方荔枝（Litchi chinensis Sonn.）进行遗传分析。
Mol Biol Rep. 2015 Jan;42(1):159-66. doi: 10.1007/s11033-014-3755-8. Epub 2014 Sep 24.
9
The Gardenia jasminoides extract and its constituent, geniposide, elicit anti-allergic effects on atopic dermatitis by inhibiting histamine in vitro and in vivo.栀子提取物及其成分栀子苷通过在体外和体内抑制组胺，对特应性皮炎产生抗过敏作用。
J Ethnopharmacol. 2014 Oct 28;156:33-40. doi: 10.1016/j.jep.2014.07.060. Epub 2014 Aug 19.
10
PCR-based identification of adriatic specimen of three scorpionfish species (Scorpaenidae, Teleostei).基于聚合酶链式反应（PCR）对三种鲉科硬骨鱼亚纲鱼类亚得里亚海标本的鉴定
Acta Biol Hung. 2014 Jun;65(2):132-43. doi: 10.1556/ABiol.65.2014.2.2.