利用改良的RAPD衍生DNA标记对大花栀子进行遗传鉴定

Genetic Authentication of Gardenia jasminoides Ellis var. grandiflora Nakai by Improved RAPD-Derived DNA Markers.

作者信息

Mei Zhiqiang, Zhou Boxu, Wei Chunli, Cheng Jingliang, Imani Saber, Chen Hanchun, Fu Junjiang

机构信息

Research Center for Preclinical Medicine, Sichuan Medical University, Luzhou 646000, China.

State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau (SAR) 999078, China.

出版信息

Molecules. 2015 Nov 10;20(11):20219-29. doi: 10.3390/molecules201119687.

The evergreen shrub, Gardenia jasminoides Ellis var. grandiflora Nakai is one of the most popular garden-plants, with significant ornamental importance. Here, we have cloned improved random ampliﬁed polymorphic DNA (RAPD) derived fragments into T-vector, and developed sequence-characterized amplified region (SCAR) markers. These markers have been deposited in GenBank database with the accession numbers KP641310, KP641311, KP641312 and KP641313 respectively. The BLAST search of database confirmed the novelty of these markers. The four SCAR markers, namely ZZH11, ZZH31, ZZH41 and ZZH51 can specifically recognize the genetic materials of G. jasminoides from other plant species. Moreover, SCAR marker ZZH31 can be used to distinguish G. jasminoides Ellis var. grandiflora Nakai from other G. jasminoides on the market. Together, this study has developed four stably molecular SCAR markers by improved RAPD-derived DNA markers for the genetic identification and authentication, and for ecological conservation of medicinal and ornamental plant G. jasminoides.

常绿灌木大花栀子（Gardenia jasminoides Ellis var. grandiflora Nakai）是最受欢迎的园林植物之一，具有重要的观赏价值。在此，我们将改良的随机扩增多态性DNA（RAPD）衍生片段克隆到T载体中，并开发了序列特征性扩增区域（SCAR）标记。这些标记已分别以登录号KP641310、KP641311、KP641312和KP641313存入GenBank数据库。数据库的BLAST搜索证实了这些标记的新颖性。四个SCAR标记，即ZZH11、ZZH31、ZZH41和ZZH51，可以特异性识别栀子与其他植物物种的遗传物质。此外，SCAR标记ZZH31可用于区分大花栀子与市场上的其他栀子。总之，本研究通过改良的RAPD衍生DNA标记开发了四个稳定的分子SCAR标记，用于药用和观赏植物栀子的遗传鉴定和认证以及生态保护。