Mei Zhiqiang, Khan Md Asaduzzaman, Yang Luquan, Yang Manman, Fu Junjiang
Indian J Exp Biol. 2015 Mar;53(3):164-9.
Gardenia jasminoides is a common garden medicinal plant known for its anticancer, anti-inflammatory, anti-thrombic, anti-fibrotic, antiviral, hepatoprotective, lung-protective, renal-protective, retina-protective and neuroprotective activities. It is found in several regions of the world, including China, but information about its genetic characteristics is limited. Here, we employed an improved method of random amplified polymorphic DNA (RAPD) analysis (with increased RAMP time) to investigate the genetic link between G. jasminoides samples collected from six different regions of Southern China. Total 26 RAPD primers were selected randomly, among which 23 primers generated reproducible polymorphic amplification bands. A total of 174 bands were obtained, where each primer had amplified 5-13 bands with an average of 7.56 bands per primer. The band size ranged approximately 150-2200 bp. Cluster dendrogram was obtained based on the improved RAPD amplification profiles, which showed that the similarity coefficients among six varieties of G. jasminoides ranged 0.67-0.88. To our knowledge, this is the first report of genetic characterization of G. jasminoides using improved RAPD analysis, which may be useful for the preservation of genetic diversity and identification of Gardenia population.
栀子是一种常见的园林药用植物，以其抗癌、抗炎、抗血栓、抗纤维化、抗病毒、保肝、保肺、保肾、保护视网膜和神经保护活性而闻名。它在世界多个地区都有发现，包括中国，但关于其遗传特征的信息有限。在这里，我们采用了一种改进的随机扩增多态性DNA（RAPD）分析方法（增加了RAPD时间）来研究从中国南方六个不同地区采集的栀子样本之间的遗传联系。随机选择了总共26个RAPD引物，其中23个引物产生了可重复的多态性扩增带。总共获得了174条带，每个引物扩增出5 - 13条带，平均每个引物7.56条带。条带大小约为150 - 2200 bp。基于改进的RAPD扩增图谱获得了聚类树状图，结果表明六个栀子品种之间的相似系数在0.67 - 0.88之间。据我们所知，这是首次使用改进的RAPD分析对栀子进行遗传特征分析的报告，这可能有助于遗传多样性的保护和栀子种群的鉴定。
