Liu Yiling, Xie Lixia, Liang Xilong, Zhang Shihong
College of Plant Sciences, Jilin University, Changchun 130062, China.
College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Int J Mol Sci. 2015 Nov 11;16(11):26978-90. doi: 10.3390/ijms161126006.
Plants synthesize and accumulate a series of stress-resistance proteins to protect normal physiological activities under adverse conditions. Chimonanthus praecox which blooms in freezing weather accumulates late embryogenesis abundant proteins (LEAs) in flowers, but C. praecox LEAs are little reported. Here, we report a group of five LEA genes of C. praecox (CpLEA5, KT727031). Prokaryotic-expressed CpLEA5 was employed in Escherichia coli to investigate bioactivities and membrane permeability at low-temperature. In comparison with the vacant strains, CpLEA5-containing strains survived in a 20% higher rate; and the degree of cell membrane damage in CpLEA5-containing strains was 55% of that of the vacant strains according to a conductivity test, revealing the low-temperature resistance of CpLEA5 in bacteria. CpLEA5 was also expressed in Pichia pastoris. Interestingly, besides low-temperature resistance, CpLEA5 conferred high resistance to salt and alkali in CpLEA5 overexpressing yeast. The CpLEA5 gene was transferred into Arabidopsis thaliana to also demonstrate CpLEA5 actions in plants. As expected, the transgenic lines were more resistant against low-temperature and drought while compared with the wild type. Taken together, CpLEA5-conferred resistances to several conditions in prokaryote and eukaryotes could have great value as a genetic technology to enhance osmotic stress and low-temperature tolerance.
植物合成并积累一系列抗逆蛋白，以在不利条件下保护正常生理活动。在寒冷天气开花的蜡梅在花朵中积累了晚期胚胎丰富蛋白（LEAs），但关于蜡梅LEAs的报道较少。在此，我们报道了一组蜡梅的五个LEA基因（CpLEA5，KT727031）。将原核表达的CpLEA5用于大肠杆菌中，以研究其在低温下的生物活性和膜通透性。与空菌株相比，含CpLEA5的菌株存活率高出20%；根据电导率测试，含CpLEA5的菌株细胞膜损伤程度为空菌株的55%，这揭示了CpLEA5在细菌中的低温抗性。CpLEA5也在毕赤酵母中表达。有趣的是，除了低温抗性外，CpLEA5在过表达酵母中还赋予了对盐碱的高抗性。将CpLEA5基因转入拟南芥中，也证明了CpLEA5在植物中的作用。正如预期的那样，与野生型相比，转基因株系对低温和干旱更具抗性。综上所述，CpLEA5在原核生物和真核生物中赋予的对多种条件的抗性，作为一种增强渗透胁迫和低温耐受性的遗传技术可能具有巨大价值。
