Derksen Merel, Mertens Vicky, Pruijn Ger J M
Department of Biomolecular Chemistry, Institute for Molecules and Materials, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands.
Department of Biomolecular Chemistry, Radboud Institute for Molecular Life Sciences, Radboud University, P.O. Box 9101, Nijmegen NL-6500 HB, The Netherlands.
Biomolecules. 2015 Nov 9;5(4):3029-50. doi: 10.3390/biom5043029.
The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence, which mimics a tRNA precursor, a well-known natural RNase P substrate, is used to target an RNA molecule for cleavage by endogenous RNase P. Alternatively, a guide sequence can be attached to M1 RNA, the (catalytic) RNase P RNA subunit of Escherichia coli. The guide sequence is specific for an RNA target, which is subsequently cleaved by the bacterial M1 RNA moiety. These approaches are applicable in both bacteria and eukaryotes. In this review, we will discuss the two technologies in which RNase P is used to reduce RNA expression levels.
核糖核酸酶P的RNA切割活性可用于降低特定RNA的水平、研究其功能，甚至根除病原体。现已开发出两种不同技术，将核糖核酸酶P用作RNA敲低工具。其中一种技术是，使用一段模仿tRNA前体（一种众所周知的天然核糖核酸酶P底物）的外部引导序列，来靶向一个RNA分子，以便由内源性核糖核酸酶P进行切割。另外，也可以将引导序列连接到M1 RNA（大肠杆菌的（催化性）核糖核酸酶P RNA亚基）上。该引导序列对一个RNA靶标具有特异性，随后该RNA靶标会被细菌的M1 RNA部分切割。这些方法在细菌和真核生物中均适用。在本综述中，我们将讨论利用核糖核酸酶P降低RNA表达水平的这两种技术。
