Homsy J J, Granger B, Krassner S M
Department of Developmental and Cell Biology, University of California, Irvine 92717.
J Protozool. 1989 Mar-Apr;36(2):150-3. doi: 10.1111/j.1550-7408.1989.tb01063.x.
Epimastigotes of Trypanosoma cruzi, Peru strain, incubated in Contreras' artificial triatomine urine transformed into metacyclic trypomastigotes within 48 h at 28 degrees C when 10 mM L-proline or L-glutamate was added to the medium. Smaller numbers of metacyclic stages were induced in the presence of glucose or L-alanine. The L-leucine and L-isoleucine, 2 amino acids known to inhibit proline catabolism, inhibited proline-induced metacyclogenesis. Cells gassed with 5% CO2 showed significantly faster rates and higher levels of transformation than those not gassed, therefore indicating the additional importance of CO2 for transformation.
将克氏锥虫秘鲁株的上鞭毛体在孔特雷拉斯人工锥蝽尿液中培养，当向培养基中添加10 mM L-脯氨酸或L-谷氨酸时，在28℃下48小时内可转化为循环后期锥鞭毛体。在葡萄糖或L-丙氨酸存在的情况下，诱导产生的循环后期阶段数量较少。已知可抑制脯氨酸分解代谢的两种氨基酸L-亮氨酸和L-异亮氨酸，抑制了脯氨酸诱导的循环后期发育。用5%二氧化碳通气的细胞比未通气的细胞显示出明显更快的速率和更高水平的转化，因此表明二氧化碳对转化具有额外的重要性。
