Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China; School of Materials, The University of Manchester, Manchester M13 9PL, UK.
Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
Int J Pharm. 2015 Dec 30;496(2):732-40. doi: 10.1016/j.ijpharm.2015.11.016. Epub 2015 Nov 10.
Curcumin (CM) possesses multiple biological activities. However, poor water solubility and low bioavailability limit its application in biomedical fields. CM nanoparticles (NPs) (230-240nm) were prepared by solution-enhanced dispersion via supercritical CO2 (SEDS) (22-22.5MPa pressure, 31-32.5°C temperature) and its biological functions were evaluated in this study. The Minimum inhibitory concentration of CM NPs against S. aureus (∼250μg/mL) was lower than CM-DMSO (∼500μg/mL). Meanwhile, CM NPs showed effective anti-oxidant ability at a concentration raging from 125 to 2000μg/mL. CM NPs showed time-dependent intracellular internalization ability, resulting in an enhanced anti-cancer effect on colorectal cancer cells (HCT116), and the mechanism could be explained by cell cycle arrest in G2/M phase associated with inducing apoptotic cells. Moreover, CM NPs exhibited reduced cytotoxicity on normal cells (NCM460) compared to CM-DMSO and 5-Fu. In conclusion, CM NPs prepared via SEDS showed potentials in biomedical applications.
姜黄素（CM）具有多种生物学活性。然而，其较差的水溶性和低生物利用度限制了其在生物医学领域的应用。本研究采用超临界 CO2 溶液强化分散（SEDS）（压力 22-22.5MPa，温度 31-32.5°C）法制备了粒径为 230-240nm 的 CM 纳米颗粒（NPs），并评价了其生物学功能。CM NPs 对金黄色葡萄球菌的最小抑菌浓度（∼250μg/mL）低于 CM-DMSO（∼500μg/mL）。同时，CM NPs 在 125-2000μg/mL 的浓度范围内表现出有效的抗氧化能力。CM NPs 表现出时间依赖性的细胞内内化能力，对结直肠癌细胞（HCT116）产生增强的抗癌作用，其机制可以通过与诱导凋亡细胞相关的 G2/M 期细胞周期停滞来解释。此外，与 CM-DMSO 和 5-Fu 相比，CM NPs 对正常细胞（NCM460）的细胞毒性降低。总之，通过 SEDS 制备的 CM NPs 在生物医学应用中具有潜力。
