Keinänen M, Bloomfield C D, Machnicki J, Griffin J D, de la Chapelle A
Department of Medical Genetics, University of Helsinki, Finland.
Leukemia. 1989 Jun;3(6):405-12.
Fresh and/or frozen bone marrow cells from five healthy individuals and seven patients with myeloid leukemia were studied using growth factors and a cytogenetic technique which allows simultaneous analysis of karotype and cell lineage. Cell lineages were identified using monoclonal antibodies in an alkaline phosphatase antialkaline phosphatase staining method. In general, cultures stimulated with a colony stimulating factor containing conditioned medium (CSF) and erythropoietin (EPO) had a higher (approximately 2-fold) mitotic index (MI) than cultures without these growth factors (maximum 7.0 vs. 3.8 after 4-day culture). The significantly higher MI in cultures with growth factors was shown to result from an increase in both erythrocytic and granulocytic-monocytic mitoses. Every culture with CSF and EPO had more erythrocytic metaphases than the identical culture without these growth factors (mean erythrocytic MI 3.1 vs. 0.3, p = 0.01 in healthy subjects; 6.9 vs. 0, p = 0.05 in leukemia). In each of the three patients showing an increased MI where lineage-specific MI was studied, the granulocytic-monocytic MI increased (mean 4.0 vs. 2.1, p = 0.05). These data suggest that growth factors increase the number of metaphases available for cytogenetic analysis from fresh or frozen marrow, and may be used to stimulate metaphases from specific lineages.
使用生长因子和一种允许同时分析核型和细胞谱系的细胞遗传学技术，对来自5名健康个体和7名髓系白血病患者的新鲜和/或冷冻骨髓细胞进行了研究。在碱性磷酸酶抗碱性磷酸酶染色方法中使用单克隆抗体来识别细胞谱系。一般来说，用含有集落刺激因子的条件培养基（CSF）和促红细胞生成素（EPO）刺激的培养物，其有丝分裂指数（MI）比没有这些生长因子的培养物更高（约2倍）（4天培养后最高分别为7.0和3.8）。结果表明，含有生长因子的培养物中显著更高的MI是由红细胞和粒细胞 - 单核细胞有丝分裂的增加导致的。每一个添加了CSF和EPO的培养物比没有这些生长因子的相同培养物有更多的红细胞中期（健康受试者中红细胞MI平均值为3.1对0.3，p = 0.01；白血病患者中为6.9对0，p = 0.05）。在研究了谱系特异性MI且MI增加的三名患者中，每一名患者的粒细胞 - 单核细胞MI都增加了（平均值为4.0对2.1，p = 0.05）。这些数据表明，生长因子增加了可用于对新鲜或冷冻骨髓进行细胞遗传学分析的中期细胞数量，并且可用于刺激特定谱系的中期细胞。
