Keinänen M, Knuutila S, Bloomfield C D, Elonen E, de la Chapelle A
Department of Medical Genetics, University of Helsinki, Finland.
Leukemia. 1987 Jan;1(1):32-7.
To determine changes in the cell lineages of metaphases karyotyped following different culture times, marrow from 11 healthy individuals was studied using a technique that allows simultaneous analysis of karyotype and cell lineage. Cell lineage was identified as erythroid by surface glycophorin A, granulocytic by Sudan black B and PM-81, and monocytic by lysozyme. Marrow examined sequentially showed granulocytic mitoses to initially decrease from a mean of 40% at 1.75 hr to 6% at 3.5 hr and then increase, being 46% by 6 hr and 82% after 1 day, and remain high for the 10 days studied. Erythroid mitoses were most frequent (mean, 72%) at 3.5 hr and then decreased rapidly, being 16% by 6 hr, 7% at 1 day, and absent thereafter. When granulocytic mitoses were least frequent, 20-36% of mitoses were also unreactive with glycophorin A. Double staining experiments to identify these cells found some to be monocytic, but most remained unidentified. The authors conclude that mitoses of different hematopoietic lineages predominate when normal marrow is studied cytogenetically at different times following aspiration, and that the major changes occur during the first 8 hours. These findings have importance for how cytogenetic studies are performed in leukemia.
为了确定不同培养时间后中期核型分析的细胞谱系变化，使用一种能够同时分析核型和细胞谱系的技术，对11名健康个体的骨髓进行了研究。细胞谱系通过表面血型糖蛋白A鉴定为红系，通过苏丹黑B和PM - 81鉴定为粒系，通过溶菌酶鉴定为单核系。连续检查的骨髓显示，粒系有丝分裂最初从1.75小时时的平均40%降至3.5小时时的6%，然后增加，6小时时为46%，1天后为82%，在研究的10天内一直保持较高水平。红系有丝分裂在3.5小时时最为频繁（平均72%），然后迅速下降，6小时时为16%，1天时为7%，此后不再出现。当粒系有丝分裂最不频繁时，20 - 36%的有丝分裂也与血型糖蛋白A无反应。通过双重染色实验来鉴定这些细胞，发现一些是单核系，但大多数仍无法鉴定。作者得出结论，在抽取骨髓后的不同时间对正常骨髓进行细胞遗传学研究时，不同造血谱系的有丝分裂占主导地位，且主要变化发生在最初的8小时内。这些发现对于白血病细胞遗传学研究的开展具有重要意义。
