Zhu Wenting, Zhang Qian, Zhang Yang, Cen Lian, Wang Jun
Department of Pediatric Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, No. 639, Zhi Zao Ju Road, Shanghai, 200011, China.
School of Chemical Engineering, East China University of Science and Technology, No. 130, Mei Long Road, Shanghai, 200237, China.
J Transl Med. 2015 Nov 14;13:357. doi: 10.1186/s12967-015-0719-2.
This study was aimed to investigate whether regeneration of periodontal ligament (PDL) like tissue could be promoted by stromal cell-derived factor-1 (SDF1) and bone morphogenetic protein-7 (BMP7) induced cell homing in delayed replantation of avulsed teeth.
Canine mandibular premolar teeth were first extracted and air-dried for 2 h followed by complete detachment of their PDL tissues. The crown and pulp of the teeth were also removed. Twenty-four roots divided into two groups (n = 12/group) were used for the following in vivo transplantation. The roots of Group A were treated with 17 % EDTA for 24 h to achieve demineralization, and then coated with SDF1 and BMP7 supplemented collagen solution. The roots of Group B were similarly treated except being coated with a pristine collagen solution. The above roots were transplanted in the sockets that formed previously during tooth extraction. At 6 months' post-operation, PDL-like tissue composed of spindle-shaped cells, capillaries and highly organized collagen fibers was observed in the interstitial space between the avulsed root surface and surrounding alveolar bone in Group A. The neo-fibers inserted deeply and perpendicularly into the cementum and adjacent bone. The periodontium-like characteristics of the neo-tissue was confirmed by immunohistochemical staining for collagen I, fibronectin and osteocalcin.
A high incidence of PDL re-establishment as 42 % was achieved for samples of Group A. However, no PDL-like tissue was found but root ankylosis and replacement resorption as well as inflammatory resorption was observed in the replanted roots of Group B.
It can be confirmed that avulsed teeth could be successfully rescued even in delayed transplantation to avoid dentoalveolar ankylosis or replacement resorption via the current developed cell homing method.
本研究旨在探讨在脱位牙延迟再植中，基质细胞衍生因子-1（SDF1）和骨形态发生蛋白-7（BMP7）诱导的细胞归巢是否能促进牙周膜（PDL）样组织的再生。
首先拔除犬下颌前磨牙，空气干燥2小时，然后将其牙周膜组织完全分离。牙齿的冠部和牙髓也被去除。将24个牙根分为两组（每组n = 12）用于以下体内移植。A组牙根用17%乙二胺四乙酸（EDTA）处理24小时以实现脱矿，然后用补充了SDF1和BMP7的胶原溶液包被。B组牙根的处理方式类似，只是用单纯的胶原溶液包被。上述牙根被移植到拔牙时预先形成的牙槽窝中。术后6个月，在A组脱位牙根表面与周围牙槽骨之间的间隙中观察到由纺锤形细胞、毛细血管和高度有序的胶原纤维组成的PDL样组织。新生纤维深深且垂直地插入牙骨质和相邻骨中。通过对I型胶原、纤连蛋白和骨钙素进行免疫组织化学染色，证实了新生组织具有牙周膜样特征。
A组样本实现了42%的高牙周膜重建发生率。然而，在B组再植牙根中未发现PDL样组织，而是观察到牙根粘连、替代性吸收以及炎性吸收。
可以确认，即使在延迟移植的情况下，通过目前开发的细胞归巢方法，脱位牙也能成功挽救，以避免牙槽骨粘连或替代性吸收。
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