Terent'ev M A, Chenin L S
Mol Gen Mikrobiol Virusol. 1989 Mar(3):10-5.
The RTF derivative of the plasmid R1drd-19 was found to stimulate recombination of the tester plasmids in a recB mutant of Escherichia coli K12. The frequency of intramolecular recombination is increased 3.5 and 20-fold, as compared to the one in rec+ and rec- strains, respectively. The frequency of interplasmid recombination is enhanced 4 and 9-fold, respectively. Considerable heterogeneity of the recombination products of the tester plasmid intramolecular recombination in recB-/RTFR1-19 strain has been revealed. It is hypothesized that a "recombinase" encoded by Rldrd-19 plasmid determines a new minor pathway in recB- (Rec P) which differs in activity and, perhaps substrate specificity from the main Rec BCD pathway.
发现质粒R1drd - 19的RTF衍生物可刺激大肠杆菌K12的recB突变体中测试质粒的重组。与rec⁺和rec⁻菌株相比，分子内重组频率分别增加了3.5倍和20倍。质粒间重组频率分别提高了4倍和9倍。已揭示recB⁻/RTFR1 - 19菌株中测试质粒分子内重组的重组产物具有相当大的异质性。据推测，由Rldrd - 19质粒编码的“重组酶”在recB⁻（Rec P）中确定了一条新的次要途径，其活性以及可能的底物特异性与主要的Rec BCD途径不同。
