Pekhov A P, Rudneva S N
Biull Eksp Biol Med. 1977 Nov;84(11):623-6.
As a result of mating of cells carrying plasmid F' lac with cells carrying plasmid F'his merodiploids carrying plasmid complex F'lacF'his were isolated. The plasmid genes of the isolated merodiploids are donated together into the recipient cells and are eliminated jointly from host-cells both spontaneously and by acridine orange. As the formation of the plasmid complex took place in E. coli cells carrying mutation in rec A gene the recombination of plasmids F' is supposed to take place in the absence of the product of gene rec. A.
携带质粒F'lac的细胞与携带质粒F'his的细胞交配，分离得到了携带质粒复合物F'lacF'his的部分二倍体。分离出的部分二倍体的质粒基因一起被转移到受体细胞中，并能自发地以及通过吖啶橙从宿主细胞中共同消除。由于质粒复合物的形成发生在rec A基因发生突变的大肠杆菌细胞中，因此推测质粒F'的重组是在rec A基因产物缺失的情况下发生的。
