Centre for Energy, Indian Institute of Technology, Guwahati, Assam 781039, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati, Assam 781039, India.
Bioresour Technol. 2016 Jan;200:770-9. doi: 10.1016/j.biortech.2015.11.004. Epub 2015 Nov 10.
Synchronized growth and neutral lipid accumulation with high lipid productivity under mixotrophic growth of the strain Chlorella sorokiniana FC6 IITG was achieved via manipulation of substrates feeding mode and supplementation of lipid elicitors in the growth medium. Screening and optimization of lipid elicitors resulted in lipid productivity of 110.59mgL(-1)day(-1) under the combined effect of lipid inducers sodium acetate and sodium chloride. Fed-batch cultivation of the strain in bioreactor with intermittent feeding of limiting nutrients and lipid inducer resulted in maximum biomass and lipid productivity of 2.08 and 0.97gL(-1)day(-1) respectively. Further, continuous production of biomass with concomitant lipid accumulation was demonstrated via continuous feeding of BG11 media supplemented with lipid inducers sodium acetate and sodium chloride. The improved biomass and lipid productivity in chemostat was found to be 2.81 and 1.27gL(-1)day(-1) respectively operated at a dilution rate of 0.54day(-1).
通过操纵基质进料方式和在生长培养基中添加脂类诱导剂，实现了混养条件下小球藻 FC6 IITG 的同步生长和中性脂积累，以及高油脂生产力。通过对脂类诱导剂的筛选和优化，在脂类诱导剂乙酸钠和氯化钠的协同作用下，油脂生产力达到 110.59mgL(-1)day(-1)。在生物反应器中进行间歇进料限制营养物和脂类诱导剂的分批培养，最高生物量和油脂生产力分别达到 2.08 和 0.97gL(-1)day(-1)。此外，通过连续补加添加了脂类诱导剂乙酸钠和氯化钠的 BG11 培养基，实现了连续生产生物质的同时进行脂类积累。在稀释率为 0.54day(-1)的情况下，在恒化器中进行的生物量和油脂生产力分别提高到 2.81 和 1.27gL(-1)day(-1)。
