Amer Nehad N, Elbahloul Yasser, Embaby Amira M, Hussein Ahmed
Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, 163 EL-Horreya Avenue, Alexandria, 21526, Egypt.
Faculty of Science, Botany and Microbiology Department, Alexandria University, Moharam Baik, Alexandria, 21511, Egypt.
World J Microbiol Biotechnol. 2017 Jul;33(7):145. doi: 10.1007/s11274-017-2305-7. Epub 2017 Jun 16.
Oleaginous microorganisms are regarded as efficient, renewable cell factories for lipid biosynthesis, a biodiesel precursor, to overwhelm the cosmopolitan energy crisis with affordable investment capital costs. Present research highlights production and characterization of lipids by a newly isolated oleaginous bacterium, Sphingomonas sp. EGY1 DSM 29616 through an eco-friendly approach. Only sweet whey [42.1% (v/v)] in tap water was efficiently used as a growth medium and lipid production medium to encourage cell growth and trigger lipid accumulation simultaneously. Cultivation of Sphingomonas sp. EGY1 DSM 29616 in shake flasks resulted in the accumulation of 8.5 g L lipids inside the cells after 36 h at 30 °C. Triglycerides of C16:C18 saturated and unsaturated fatty acids showed a similar pattern to tripalmitin or triolein; deduced from gas chromatography (GC), thin layer chromatography (TLC), and Matrix-assisted laser desorption/ionization time-of-flight-mass spectra analysis (MALDI-TOF-MS) analyses. Batch cultivation 2.5 L in a laboratory scale fermenter led to 13.8 g L accumulated lipids after 34 h at 30 °C. Present data would underpin the potential of Sphingomonas sp. EGY1 DSM 29616 as a novel renewable cell factory for biosynthesis of biodiesel.
产油微生物被视为用于脂质生物合成（生物柴油前体）的高效、可再生细胞工厂，可通过合理的投资资本成本来应对全球能源危机。目前的研究重点是通过一种新分离的产油细菌——鞘氨醇单胞菌属（Sphingomonas sp.）EGY1 DSM 29616，采用环保方法生产和表征脂质。仅将自来水中的甜乳清[42.1%（v/v）]有效地用作生长培养基和脂质生产培养基，以同时促进细胞生长并引发脂质积累。在30℃下于摇瓶中培养鞘氨醇单胞菌属EGY1 DSM 29616，36小时后细胞内积累了8.5 g/L脂质。通过气相色谱（GC）、薄层色谱（TLC）和基质辅助激光解吸/电离飞行时间质谱分析（MALDI-TOF-MS）分析推断，C16:C18饱和与不饱和脂肪酸的甘油三酯显示出与三棕榈精或三油精相似的模式。在实验室规模的发酵罐中进行2.5 L分批培养，30℃下34小时后积累了13.8 g/L脂质。目前的数据将支持鞘氨醇单胞菌属EGY1 DSM 29616作为生物柴油生物合成新型可再生细胞工厂的潜力。
