CXCR4表达与食管鳞状细胞癌患者的不良预后相关。

CXCR4 Expression is Associated with Poor Prognosis in Patients with Esophageal Squamous Cell Carcinoma.

作者信息

Goto Masakazu, Yoshida Takahiro, Yamamoto Yota, Furukita Yoshihito, Inoue Seiya, Fujiwara Satoshi, Kawakita Naoya, Nishino Takeshi, Minato Takuya, Yuasa Yasuhiro, Yamai Hiromichi, Takechi Hirokazu, Seike Junichi, Bando Yoshimi, Tangoku Akira

机构信息

Department of Thoracic, Endocrine Surgery and Oncology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.

Sainokuni Higashiomiya Medical Center, Saitama, Japan.

出版信息

Ann Surg Oncol. 2017 Mar;24(3):832-840. doi: 10.1245/s10434-015-4974-5. Epub 2015 Nov 17.

DOI:10.1245/s10434-015-4974-5

PMID:26577115

Abstract

BACKGROUND

Chemokines and their receptors are known to play important roles in the tumorigenesis of many malignancies. The chemokine CXCL12 and its receptors CXCR4 and CXCR7 were suggested to be involved in cancer invasion and metastasis. The aim of this retrospective study was to evaluate the prognostic impact of the expressions of CXCL12, CXCR4 and CXCR7 in patients with esophageal squamous cell carcinoma (ESCC).

METHODS

We used immunohistochemistry (IHC) and reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate the expressions of CXCL12, CXCR4, and CXCR7 in ESCC patients' tumor biopsy specimens obtained during preoperative endoscopy or surgery. These results were compared with the patients' clinicopathological parameters and survival.

RESULTS

IHC was conducted for 172 patients. High expression of CXCR4 in the cytoplasm and nuclei and that of CXCR7 were associated with poor cause-specific survival (CSS) (P= .002 and .010, respectively). The specimens from 52 of the 172 patients were examined by RT-PCR and quantitative real-time PCR. The expression levels of messenger RNA (mRNA) of CXCR4 and CXCR7 were significantly increased in the tumors compared with normal esophageal mucosae (P < .0001). The expression level of mRNA of CXCR4 was associated with poor recurrence-free survival and CSS (P = .012 and .038, respectively).

CONCLUSIONS

CXCR4 expression is associated with poor prognosis in patients with ESCC.

摘要

背景

已知趋化因子及其受体在许多恶性肿瘤的发生发展中起重要作用。趋化因子CXCL12及其受体CXCR4和CXCR7被认为与癌症侵袭和转移有关。本回顾性研究的目的是评估CXCL12、CXCR4和CXCR7的表达对食管鳞状细胞癌（ESCC）患者预后的影响。

方法

我们使用免疫组织化学（IHC）和逆转录聚合酶链反应（RT-PCR）来评估在术前内镜检查或手术期间获取的ESCC患者肿瘤活检标本中CXCL12、CXCR4和CXCR7的表达。将这些结果与患者的临床病理参数和生存率进行比较。

结果

对172例患者进行了免疫组织化学检测。CXCR4在细胞质和细胞核中的高表达以及CXCR7的高表达与特定病因生存率（CSS）差相关（分别为P = .002和.010）。对172例患者中的52例标本进行了RT-PCR和定量实时PCR检测。与正常食管黏膜相比，肿瘤中CXCR4和CXCR7的信使核糖核酸（mRNA）表达水平显著升高（P < .0001）。CXCR4的mRNA表达水平与无复发生存率和CSS差相关（分别为P = .012和.038）。