Wang Ming-Qi, Zhu Wen-Xiang, Song Zhuan-Zhuan, Li Shuo, Zhang Yong-Zhao
School of Pharmacy, Jiangsu University, Zhenjiang 212013, PR China
Bioorg Med Chem Lett. 2015 Dec 15;25(24):5672-6. doi: 10.1016/j.bmcl.2015.11.007.
In this Letter, three triphenylamine-based dyes (TPA-1, TPA-2a and TPA-2b) with donor–bridge–acceptor (D–p–A) structure were designed and synthesized for the purpose of G-quadruplexes recognition. In aqueous conditions, the interactions of the dyes with G-quadruplexes were studied with the aim to establish the influence of the geometry of the dyes on their binding and probing properties. Results indicate that TPA-2b displays significant selective colorimetric and fluorescent changes upon binding of G-quadruplex DNA. More importantly, its distinct color change enables visual detection and differentiation of G-quadruplexes from single and duplex DNA structures. CD titration date reveals that TPA-2b could induce and stabilize the formation of G-quadruplex structure. All these remarkable properties of TPA-2b suggest that it should have promising application in the field of G-quadruplexes research.
在本信函中，为实现对G-四链体的识别，设计并合成了三种具有给体-桥-受体（D–p–A）结构的三苯胺基染料（TPA-1、TPA-2a和TPA-2b）。在水性条件下，研究了这些染料与G-四链体的相互作用，旨在确定染料几何结构对其结合和探测特性的影响。结果表明，TPA-2b在与G-四链体DNA结合时表现出显著的选择性比色和荧光变化。更重要的是，其明显的颜色变化能够实现对G-四链体与单链和双链DNA结构的视觉检测和区分。圆二色光谱滴定数据表明，TPA-2b能够诱导并稳定G-四链体结构的形成。TPA-2b的所有这些显著特性表明，它在G-四链体研究领域应具有广阔的应用前景。
