从胰岛素和胰岛素样生长因子-I受体的天然和重组蛋白到重组单链抗体的亲和色谱法。
Affinity Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies.
作者信息
Fujita-Yamaguchi Yoko
机构信息
Department of Diabetes Complications and Metabolism, Beckman Research Institute of City of Hope , Duarte, CA , USA.
出版信息
Front Endocrinol (Lausanne). 2015 Oct 27;6:166. doi: 10.3389/fendo.2015.00166. eCollection 2015.
Abstract
Affinity chromatography is an efficient method to isolate proteins by taking advantage of their affinities for specific molecules such as substrates, inhibitors, antigens, ligands, antibodies, and other interacting molecules, including subunits. Nowadays, we take the effectiveness and excellence of this technology for granted. This essay will mainly cover the use of affinity chromatography based on my experience.
摘要
亲和层析是一种通过利用蛋白质对特定分子（如底物、抑制剂、抗原、配体、抗体以及其他相互作用分子，包括亚基）的亲和力来分离蛋白质的有效方法。如今，我们认为这项技术的有效性和卓越性是理所当然的。本文将根据我的经验，主要介绍亲和层析的应用。
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