Founds Sandra, Zeng Xuemei, Lykins David, Roberts James M
School of Nursing and Magee-Womens Research Institute, University of Pittsburgh, 3500 Victoria St. 448 VB, Pittsburgh, PA 15261, USA.
Biomedical Mass Spectrometry Center Schools of the Health Sciences, University of Pittsburgh, Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, PA 15261, USA.
Int J Mol Sci. 2015 Nov 13;16(11):27208-27. doi: 10.3390/ijms161126023.
The potential for developing molecules of interest in preclinical preeclampsia from candidate genes that were discovered on gene expression microarray analysis has been challenged by limited access to additional first trimester trophoblast and decidual tissues. The question of whether these candidates encode secreted proteins that may be detected in maternal circulation early in pregnancy has been investigated using various proteomic methods. Pilot studies utilizing mass spectrometry based proteomic assays, along with enzyme linked immunosorbent assays (ELISAs), and Western immunoblotting in first trimester samples are reported. The novel targeted mass spectrometry methods led to robust multiple reaction monitoring assays. Despite detection of several candidates in early gestation, challenges persist. Future antibody-based studies may lead to a novel multiplex protein panel for screening or detection to prevent or mitigate preeclampsia.
基因表达微阵列分析中发现的候选基因用于开发临床前子痫前期相关感兴趣分子的潜力，因难以获取更多孕早期滋养层和蜕膜组织而受到挑战。人们使用各种蛋白质组学方法研究了这些候选基因是否编码可在孕期早期母体循环中检测到的分泌蛋白。本文报道了利用基于质谱的蛋白质组学检测、酶联免疫吸附测定（ELISA）以及孕早期样本的Western免疫印迹法开展的初步研究。新型靶向质谱方法带来了可靠的多反应监测检测。尽管在妊娠早期检测到了几种候选物，但挑战依然存在。未来基于抗体的研究可能会带来一种用于筛查或检测的新型多重蛋白质组，以预防或减轻子痫前期。
