通过全基因组测序加强对22型地方性耐甲氧西林金黄色葡萄球菌IV型菌株在患者和环境场所中院内传播的追踪。
Enhanced Tracking of Nosocomial Transmission of Endemic Sequence Type 22 Methicillin-Resistant Staphylococcus aureus Type IV Isolates among Patients and Environmental Sites by Use of Whole-Genome Sequencing.
作者信息
Kinnevey Peter M, Shore Anna C, Mac Aogáin Micheál, Creamer Eilish, Brennan Gráinne I, Humphreys Hilary, Rogers Thomas R, O'Connell Brian, Coleman David C
机构信息
Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland.
Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.
出版信息
J Clin Microbiol. 2016 Feb;54(2):445-8. doi: 10.1128/JCM.02662-15. Epub 2015 Nov 18.
Abstract
Whole-genome sequencing (WGS) of 41 patient and environmental sequence type 22 methicillin-resistant Staphylococcus aureus staphylococcal cassette chromosome mec type IV (ST22-MRSA-IV) isolates recovered over 6 weeks in one acute hospital ward in Dublin, Ireland, where ST22-MRSA IV is endemic, revealed 228 pairwise combinations differing by <40 single nucleotide variants corresponding to potential cross-transmission events (CTEs). In contrast, 15 pairwise combinations of isolates representing five CTEs were previously identified by conventional molecular epidemiological typing. WGS enhanced ST22-MRSA-IV tracking and highlighted potential transmission of MRSA via the hospital environment.
摘要
对在爱尔兰都柏林一家急症医院病房6周内分离出的41株患者和环境来源的序列型22耐甲氧西林金黄色葡萄球菌葡萄球菌盒式染色体mec IV型（ST22-MRSA-IV）菌株进行全基因组测序，结果显示有228对组合，其单核苷酸变异少于40个，对应潜在的交叉传播事件（CTE）。相比之下，通过传统分子流行病学分型先前已鉴定出代表5个CTE的15对菌株组合。全基因组测序加强了对ST22-MRSA-IV的追踪，并突出了耐甲氧西林金黄色葡萄球菌通过医院环境的潜在传播。
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