Qian Chunxiang, Chen Huaicheng, Ren Lifu, Luo Mian
School of Materials Science and Engineering, Southeast University Nanjing, China ; Research Institute of Green Construction Materials, Southeast University Nanjing, China.
Front Microbiol. 2015 Nov 4;6:1225. doi: 10.3389/fmicb.2015.01225. eCollection 2015.
This research investigated the self-healing potential of early age cracks in cement-based materials incorporating the bacteria which can produce carbonic anhydrase. Cement-based materials specimens were pre-cracked at the age of 7, 14, 28, 60 days to study the repair ability influenced by cracking time, the width of cracks were between 0.1 and 1.0 mm to study the healing rate influenced by width of cracks. The experimental results indicated that the bacteria showed excellent repairing ability to small cracks formed at early age of 7 days, cracks below 0.4 mm was almost completely closed. The repair effect reduced with the increasing of cracking age. Cracks width influenced self-healing effectiveness significantly. The transportation of CO2and Ca(2+) controlled the self-healing process. The computer simulation analyses revealed the self-healing process and mechanism of microbiologically precipitation induced by bacteria and the depth of precipitated CaCO3 could be predicted base on valid Ca(2+).
本研究调查了掺入能产生碳酸酐酶的细菌的水泥基材料中早期裂缝的自愈潜力。对水泥基材料试件在7天、14天、28天、60天龄期进行预开裂,以研究开裂时间对修复能力的影响,裂缝宽度在0.1至1.0毫米之间,以研究裂缝宽度对愈合率的影响。实验结果表明,该细菌对7天早期形成的小裂缝显示出优异的修复能力,0.4毫米以下的裂缝几乎完全闭合。修复效果随开裂龄期的增加而降低。裂缝宽度对自愈效果有显著影响。二氧化碳和钙离子的传输控制着自愈过程。计算机模拟分析揭示了细菌诱导的微生物沉淀的自愈过程和机理,并且基于有效的钙离子可以预测碳酸钙沉淀的深度。