Bang S S., Galinat J K., Ramakrishnan V
Department of Chemistry and Chemical Engineering, South Dakota School of Mines and Technology, Rapid City, SD, USA
Enzyme Microb Technol. 2001 Mar 8;28(4-5):404-409. doi: 10.1016/s0141-0229(00)00348-3.
Polyurethane (PU) foam was used to immobilize the whole cell of Bacillus pasteurii. The immobilized cells exhibited the rates of calcite precipitation and ammonia production as high as those of the free cells. Scanning electron micrographs identified the cells embedded in calcite crystals throughout PU matrices. Calcite in PU showed little effect on the elastic modulus and tensile strength of the polymer, but increased the compressive strengths of concrete cubes, whose cracks were remediated with PU-immobilized cells. These observations led us to believe that the calcite might remain as a form of precipitation, not as a bonding material within the matrices.
聚氨酯（PU）泡沫被用于固定巴氏芽孢杆菌的全细胞。固定化细胞的方解石沉淀速率和氨生成速率与游离细胞相当。扫描电子显微镜照片显示，在整个PU基质中，细胞嵌入方解石晶体中。PU中的方解石对聚合物的弹性模量和拉伸强度影响不大，但提高了混凝土立方体的抗压强度，其裂缝用PU固定化细胞进行了修复。这些观察结果使我们相信，方解石可能以沉淀的形式存在，而不是作为基质中的粘结材料。
