完全断奶的宫内生长迟缓仔猪的抗氧化能力及氧化还原活性微量矿物质浓度
Antioxidant capacity and concentration of redox-active trace mineral in fully weaned intra-uterine growth retardation piglets.
作者信息
Zhang Hao, Li Yue, Wang Tian
机构信息
College of Animal Science and Technology, Nanjing Agricultural University, Weigang 1, Nanjing, 210095 China.
出版信息
J Anim Sci Biotechnol. 2015 Nov 19;6:48. doi: 10.1186/s40104-015-0047-7. eCollection 2015.
BACKGROUND
The redox status of intra-uterine growth retardation (IUGR) piglets post-weaning has been poorly studied.
METHODS
Newborns from twenty-four sows were weighted, weaned at 21 d and fed a starter diet until sampling. Sampling was done at 14 d post-weaning. A piglet was defined as IUGR when its birth weight was 2 SD below the mean birth weight of the total population. At weaning, eighteen piglets with nearly equal body weight from each category (i.e. IUGR or normal birth weight (NBW) piglets) were selected and then allocated to two treatments, consisted of six replicates with each pen having three piglets.
RESULTS
Compared with NBW group, IUGR significantly decreased average daily gain (P < 0.001), average daily feed intake (P = 0.003), and feed efficiency (P < 0.001) of piglets during the first two weeks post-weaning. IUGR decreased the activities of total antioxidant capacity (P = 0.019), total superoxide dismutase (T-SOD, P = 0.023), and ceruloplasmin (P = 0.044) but increased the levels of malondialdehyde (P = 0.040) and protein carbonyl (P = 0.010) in plasma. Similarly, the decreased activities of T-SOD (P = 0.005), copper- and zinc-containing superoxide dismutase (Cu/Zn-SOD, P = 0.002), and catalase (P = 0.049) was observed in the liver of IUGR piglets than these of NBW piglets. IUGR decreased hepatic Cu/Zn-SOD activity (P = 0.023) per unit of Cu/Zn-SOD protein in piglets when compared with NBW piglets. In addition, IUGR piglets exhibited the decreases in accumulation of copper in both plasma (P = 0.001) and liver (P = 0.014), as well as the concentrations of iron (P = 0.002) and zinc (P = 0.048) in liver. Compared with NBW, IUGR down-regulated mRNA expression of Cu/Zn-SOD (P = 0.021) in the liver of piglets.
CONCLUSIONS
The results indicated that IUGR impaired antioxidant capacity and resulted in oxidative damage in fully weaned piglets, which might be associated with the decreased levels of redox-active trace minerals. This study highlights the importance of redox status in IUGR offspring and provides a rationale for alleviating oxidative damage by dietary interventions aiming to supplement trace minerals and to restore redox balance in the future.
背景
断奶后宫内生长受限（IUGR）仔猪的氧化还原状态研究较少。
方法
对24头母猪所产的新生仔猪进行称重，21日龄断奶并饲喂开食料直至采样。在断奶后14天进行采样。当仔猪出生体重低于总体平均出生体重2个标准差时，将其定义为IUGR。断奶时，从每个类别（即IUGR或正常出生体重（NBW）仔猪）中选择体重相近的18头仔猪，然后分为两种处理，每种处理6个重复，每栏3头仔猪。
结果
与NBW组相比，IUGR显著降低了断奶后前两周仔猪的平均日增重（P < 0.001）、平均日采食量（P = 0.003）和饲料效率（P < 0.001）。IUGR降低了血浆中总抗氧化能力（P = 0.019）、总超氧化物歧化酶（T-SOD，P = 0.023）和铜蓝蛋白（P = 0.044）的活性，但增加了丙二醛（P = 0.040）和蛋白质羰基（P = 0.010）的水平。同样，与NBW仔猪相比，IUGR仔猪肝脏中T-SOD（P = 0.005）、铜锌超氧化物歧化酶（Cu/Zn-SOD，P = 0.002）和过氧化氢酶（P = 0.049）的活性降低。与NBW仔猪相比，IUGR仔猪每单位Cu/Zn-SOD蛋白的肝脏Cu/Zn-SOD活性降低（P = 0.023）。此外，IUGR仔猪血浆（P = 0.001）和肝脏（P = 0.014）中的铜积累以及肝脏中铁（P = 0.002）和锌（P = 0.048）的浓度均降低。与NBW相比，IUGR下调了仔猪肝脏中Cu/Zn-SOD的mRNA表达（P = 0.021）。
结论
结果表明，IUGR损害了完全断奶仔猪的抗氧化能力并导致氧化损伤，这可能与氧化还原活性微量元素水平降低有关。本研究强调了IUGR后代氧化还原状态的重要性，并为未来通过旨在补充微量元素和恢复氧化还原平衡的饮食干预来减轻氧化损伤提供了理论依据。
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