School of Karst Science, Guizhou Normal University, No. 116 North Baoshan Road, Yunyan District, Guiyang 550001, China.
State Engineering Technology Institute for Karst Desertification Control, No. 116 North Baoshan Road, Yunyan District, Guiyang 550001, China.
Oxid Med Cell Longev. 2022 Mar 19;2022:2644205. doi: 10.1155/2022/2644205. eCollection 2022.
The aim of the present study was to investigate the effects of intrauterine growth retardation (IUGR) on the intestinal morphology, intestinal epithelial cell apoptosis, intestinal antioxidant capacity, intestinal glucose absorption capacity, and intestinal barrier function of piglets during the suckling period. A total of eight normal-birth-weight (NBW) piglets and eight IUGR newborn piglets (Duroc × Landrace × Yorkshire) were selected from eight litters, one NBW and one IUGR newborn piglet per litter. In each litter, piglets with birth weight of 1.54 ± 0.04 kg (within one SD of the mean birth weight) were selected as NBW piglets and piglets with birth weight of 0.82 ± 0.03 kg (two SD below the mean birth weight) were selected as IUGR piglets. At 21 days of age, all piglets were killed by exsanguinations for sampling. The results showed the body weight (BW) of IUGR piglets on day 0, day 7, day 14, and day 21, and the body weight gain (BWG) of IUGR piglets was significantly lower than that of NBW piglets. IUGR piglets exhibited impaired intestinal morphology, raised enterocyte apoptosis, and increased oxidative damage. It showed that IUGR leads to a lower antioxidant capacity and glucose absorption in the jejunum. In accordance, IUGR caused the intestinal barrier dysfunction by impairing tight junctions and increasing intestinal inflammatory injury. Collectively, these results add to our understanding that IUGR affects intestinal health of suckling piglets via altering intestinal antioxidant capacity, glucose uptake, tight junction, and immune responses, and the slow growth of piglets with IUGR may be associated with intestinal injury.
本研究旨在探讨宫内生长迟缓（IUGR）对哺乳期仔猪肠道形态、肠上皮细胞凋亡、肠道抗氧化能力、肠道葡萄糖吸收能力和肠道屏障功能的影响。从 8 窝正常出生体重（NBW）仔猪和 8 窝 IUGR 新生仔猪（杜洛克×长白×约克夏）中分别选择了 8 头 NBW 和 8 头 IUGR 新生仔猪（每窝 1 头 NBW 和 1 头 IUGR）。在每窝仔猪中，选择出生体重为 1.54 ± 0.04kg（出生体重平均值的 1 个标准差内）的仔猪为 NBW 仔猪，选择出生体重为 0.82 ± 0.03kg（出生体重平均值的 2 个标准差以下）的仔猪为 IUGR 仔猪。在 21 日龄时，所有仔猪均通过放血处死以进行采样。结果显示，IUGR 仔猪在 0 日龄、7 日龄、14 日龄和 21 日龄的体重（BW）以及 IUGR 仔猪的体重增加（BWG）显著低于 NBW 仔猪。IUGR 仔猪的肠道形态受损，肠上皮细胞凋亡增加，氧化损伤加重。这表明 IUGR 导致空肠抗氧化能力和葡萄糖吸收能力降低。相应地，IUGR 通过破坏紧密连接和增加肠道炎症损伤导致肠道屏障功能障碍。总之，这些结果表明，IUGR 通过改变肠道抗氧化能力、葡萄糖摄取、紧密连接和免疫反应来影响哺乳期仔猪的肠道健康，而 IUGR 仔猪的生长缓慢可能与肠道损伤有关。
