David M, Katzenelson V, Hazaz B, Ben-Chetrit A, Sandbank M
Department of Dermatology, Beilinson Medical Center, Petah Tiqva, Israel.
Arch Dermatol. 1989 Jun;125(6):787-90.
IgG subclasses were determined in perilesional skin of 13 patients with pemphigus with active disease and of 14 patients in a state of clinical remission. Using indirect immunofluorescence technique, frozen sections were incubated with mouse monoclonal antihuman IgG1, IgG2, IgG3, and IgG4 followed by a second incubation with fluorescein isothiocyanate-conjugated goat antimouse IgG. The results showed that among patients with active disease, IgG1 was found in all of them and IgG4 in 85%, while IgG2 and IgG3 were found in 54% and 77%, respectively. For patients in remission the most common subclass was IgG4 in 79% of patients, and in a decreased order IgG1, 50%; IgG3, 29%; and IgG2, 14%. It appears that IgG1 and IgG4 are predominant among patients with active lesions. IgG1 seems to be the most sensitive indicator for activity of the disease. IgG4, normally found in the lowest concentration in human serum, is the most common subclass in patients who are in remission. IgG3 and C3 may have a predictive value for remission.
对13例患有活动性疾病的天疱疮患者和14例处于临床缓解状态的患者的皮损周围皮肤进行了IgG亚类检测。采用间接免疫荧光技术，将冰冻切片与小鼠单克隆抗人IgG1、IgG2、IgG3和IgG4孵育，然后再与异硫氰酸荧光素偶联的山羊抗小鼠IgG进行二次孵育。结果显示，在患有活动性疾病的患者中，全部患者均检测到IgG1，85%的患者检测到IgG4，而IgG2和IgG3的检出率分别为54%和77%。对于处于缓解期的患者，最常见的亚类是IgG4，占79%，其次是IgG1，占50%；IgG3，占29%；IgG2，占14%。看来在有活动性皮损的患者中IgG1和IgG4占主导地位。IgG1似乎是疾病活动最敏感的指标。IgG4通常在人血清中浓度最低，是处于缓解期患者中最常见的亚类。IgG3和C3可能对缓解有预测价值。
