Roan Esra, Wilhelm Kristina R, Waters Christopher M
Biomedical Engineering Department, University of Memphis, Memphis, Tennessee.
Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee.
Biophys J. 2015 Nov 17;109(10):2051-7. doi: 10.1016/j.bpj.2015.10.005.
Epithelial cell migration during wound repair involves a complex interplay of intracellular processes that enable motility while preserving contact among the cells. Recent evidence suggests that fluctuations of the intracellular biophysical state of cells generate traction forces at the basal side of the cells that are necessary for the cells to migrate. However, less is known about the biophysical and structural changes throughout the cells that accompany these fluctuations. Here, we utilized, to our knowledge, a novel kymographic nanoindentation method to obtain spatiotemporal measurements of the elastic moduli of living cells during migration after wounding. At the onset of migration, the elastic modulus increased near the migration front. In addition, the intensity of fluctuations in the elastic modulus changed at the migration front, and these changes were dependent upon f-actin, one of the major components of the cytoskeleton. These results demonstrate the unique biophysical changes that occur at the onset of migration as cells transition from a stationary to a migratory state.
伤口修复过程中的上皮细胞迁移涉及细胞内多种复杂的相互作用过程，这些过程既能使细胞具有运动能力，又能保持细胞间的接触。最近的证据表明，细胞内生物物理状态的波动会在细胞基底侧产生牵引力，这是细胞迁移所必需的。然而，对于伴随这些波动的整个细胞内的生物物理和结构变化，我们了解得还较少。在此，据我们所知，我们采用了一种新型的波形记录纳米压痕方法，以获取伤口愈合后细胞迁移过程中活细胞弹性模量的时空测量数据。在迁移开始时，迁移前沿附近的弹性模量增加。此外，弹性模量波动的强度在迁移前沿发生了变化，并且这些变化依赖于细胞骨架的主要成分之一——丝状肌动蛋白（f-肌动蛋白）。这些结果证明了细胞从静止状态转变为迁移状态时，在迁移开始阶段发生的独特生物物理变化。
