Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Department of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, TX 79409, USA.
Sci Total Environ. 2016 Feb 1;543(Pt A):267-273. doi: 10.1016/j.scitotenv.2015.10.165. Epub 2015 Nov 14.
Etheno-DNA adducts are biomarkers for assessing oxidative stress. In this study, the aim was to detect the level of etheno-DNA adducts and explore the relationship between the etheno-DNA adducts and genotoxicity biomarkers of the diesel engine exhaust (DEE)-exposed workers. We recruited 86 diesel engine testing workers with long-term exposure to DEE and 99 non-DEE-exposed workers. The urinary mono-hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) and etheno-DNA adducts (εdA and εdC) were detected by HPLC-MS/MS and UPLC-MS/MS, respectively. Genotoxicity biomarkers were also evaluated by comet assay and cytokinesis-block micronucleus assay. The results showed that urinary εdA was significantly higher in the DEE-exposed workers (p<0.001), exhibited 2.1-fold increase compared with the non-DEE-exposed workers. The levels of urinary OH-PAHs were positively correlated with the level of εdA among all the study subjects (p<0.001). Moreover, we found that the increasing level of εdA was significantly associated with the increased olive tail moment, percentage of tail DNA, or frequency of micronucleus in the study subjects (p<0.01). No significant association was observed between the εdC level and any measured genotoxicity biomarkers. In summary, εdA could serve as an indicator for DEE exposure in the human population.
乙基亚硝胺 DNA 加合物是评估氧化应激的生物标志物。本研究旨在检测乙基亚硝胺 DNA 加合物的水平，并探讨其与柴油机尾气（DEE）暴露工人的遗传毒性生物标志物之间的关系。我们招募了 86 名长期接触 DEE 的柴油机测试工人和 99 名非 DEE 暴露工人。通过高效液相色谱-串联质谱法（HPLC-MS/MS）和超高效液相色谱-串联质谱法（UPLC-MS/MS）分别检测了尿液中单羟基多环芳烃（OH-PAHs）和乙基亚硝胺 DNA 加合物（εdA 和 εdC）。还通过彗星试验和细胞有丝分裂阻断微核试验评估了遗传毒性生物标志物。结果表明，DEE 暴露工人的尿液 εdA 显著升高（p<0.001），与非 DEE 暴露工人相比，其值增加了 2.1 倍。所有研究对象的尿液 OH-PAHs 水平与 εdA 水平呈正相关（p<0.001）。此外，我们发现 εdA 水平的升高与研究对象中橄榄拖尾矩、尾部 DNA 百分比或微核频率的增加显著相关（p<0.01）。εdC 水平与任何测量的遗传毒性生物标志物之间均无显著关联。总之，εdA 可作为人群中 DEE 暴露的指标。
