酿酒酵母中的α-凝集素表达

Alpha-agglutinin expression in Saccharomyces cerevisiae.

作者信息

Wojciechowicz D, Lipke P N

机构信息

Department of Biological Sciences, Hunter College, New York 10021.

出版信息

Biochem Biophys Res Commun. 1989 May 30;161(1):46-51. doi: 10.1016/0006-291x(89)91557-x.

DOI:10.1016/0006-291x(89)91557-x

PMID:2659000

Abstract

A polyclonal antiserum raised against purified alpha-agglutinin was made specific for alpha-agglutinin after adsorption with a cells. The adsorbed antiserum identified alpha-agglutinin peptides on Western blots and bound to cell surface alpha-agglutinin, inhibiting the binding of alpha cells to a cells. Using the antibody, we have determined that 1) the surface distribution of alpha-agglutinin on alpha cells is polar, 2) about 5 x 10(4) molecules/cell are constitutively expressed on strain X2180-1B (alpha) cells, and 3) treatment of alpha cells with the sex pheromone a-factor causes an increase in cell surface alpha-agglutinin, consistent with the a-factor induced increase in cell agglutinability.

摘要

用纯化的α-凝集素制备的多克隆抗血清，在用a细胞吸附后对α-凝集素具有特异性。吸附后的抗血清在蛋白质免疫印迹法中鉴定出α-凝集素肽，并与细胞表面的α-凝集素结合，抑制α细胞与a细胞的结合。使用该抗体，我们确定：1）α-凝集素在α细胞上的表面分布是极性的；2）在X2180-1B（α）菌株细胞上组成性表达约5×10⁴个分子/细胞；3）用性信息素a因子处理α细胞会导致细胞表面α-凝集素增加，这与a因子诱导的细胞凝集性增加一致。