Yamamoto Y, Taniyama Y, Kikuchi M
Protein Engineering Research Institute, Osaka, Japan.
Biochemistry. 1989 Mar 21;28(6):2728-32. doi: 10.1021/bi00432a054.
To elucidate the role of the proline residue in the engineered signal sequence that directs the secretion of human lysozyme in Saccharomyces cerevisiae, we have remodeled an idealized signal sequence L8 = Met-Arg-(Leu)8-Pro-Leu-Ala-Ala-Leu-Gly [Yamamoto, Y., Taniyama, Y., Kikuchi, M., & Ikehara, M. (1987) Biochem. Biophys. Res. Commun. 149, 431-436] in the vicinity of the proline residue. By analyzing the secretory capability of 10 engineered signal sequences, we have shown the following. (1) The proline residue is important for the secretion of human lysozyme and is allowed at position -4, -5, or -6. (2) The secretory capability of the engineered signal sequences is correlated with their predicted conformations. (3) The functional signal sequences that we have investigated can be generalized as follows: Met-Arg-(Leu)n-Pro-(Xaa)-Ala-Leu-Gly where n equals 6-12 and Xaa is Leu, Ala, or Leu-Ala or can be omitted.
为阐明脯氨酸残基在指导人溶菌酶在酿酒酵母中分泌的工程化信号序列中的作用，我们在脯氨酸残基附近对理想化信号序列L8 = Met-Arg-(Leu)8-Pro-Leu-Ala-Ala-Leu-Gly [山本洋, 谷山洋, 菊池正, & 池原正. (1987) Biochem. Biophys. Res. Commun. 149, 431 - 436] 进行了重塑。通过分析10个工程化信号序列的分泌能力，我们得到了以下结果。(1) 脯氨酸残基对人溶菌酶的分泌很重要，且允许位于-4、-5或-6位。(2) 工程化信号序列的分泌能力与其预测构象相关。(3) 我们所研究的功能性信号序列可归纳如下：Met-Arg-(Leu)n-Pro-(Xaa)-Ala-Leu-Gly，其中n等于6 - 12，Xaa为Leu、Ala或Leu-Ala，或者可以省略。
