Yamamoto Y, Kikuchi M
Protein Engineering Research Institute, Osaka, Japan.
Eur J Biochem. 1989 Sep 1;184(1):233-6. doi: 10.1111/j.1432-1033.1989.tb15011.x.
Recently, we reported that the synthesis of human lysozyme in yeast is inhibited when artificially designed non-native signal sequences are used [Yamamoto et al. (1987) Biochem. Biophys. Res. Commun. 149, 431-436; (1989) Biochemistry 28, 2728-2732]. Pulse/chase experiments described in the present paper have now revealed that precursor lysozymes are actually synthesized with these signal sequences, but that they are spontaneously degraded, suggesting that the enzyme is destabilized by the signal sequences. The data further show a good correlation between the secretory capability of the artificial signal sequences and the efficiency of the processing of precursor lysozyme.
最近，我们报道了使用人工设计的非天然信号序列时，酵母中人类溶菌酶的合成受到抑制[山本等人（1987年）《生物化学与生物物理学研究通讯》149，431 - 436；（1989年）《生物化学》28，2728 - 2732]。本文所述的脉冲/追踪实验现已表明，前体溶菌酶实际上是与这些信号序列一起合成的，但它们会自发降解，这表明该酶因信号序列而不稳定。数据还进一步显示了人工信号序列的分泌能力与前体溶菌酶加工效率之间的良好相关性。
