人成纤维细胞重编程中病毒转导与游离型转染效率的比较
Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming.
作者信息
Vdovin A S, Lupatov A Yu, Kholodenko I V, Yarygin K N
机构信息
V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2015 Nov;160(1):123-8. doi: 10.1007/s10517-015-3112-5. Epub 2015 Nov 22.
Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector: ~200 and 8 colonies per 10(5) cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60.
通过两种重编程方法从成人皮肤成纤维细胞中获得了诱导多能干细胞。用含有oriP/EBNA-1序列的载体进行游离型转染以递送重编程基因Oct4、Sox2、Klf4、L-Myc和Lin28，结果证明其比用仙台病毒载体进行病毒转导更有效：在培养第21天时，每10⁵个细胞中分别发现约200个和8个克隆。通过这两种方法获得的诱导多能干细胞克隆表达多能性标志物Tra1-60。
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