Valuev L I, Valuev I L, Vanchugova L V, Valueva T A
Prikl Biokhim Mikrobiol. 2015 Sep-Oct;51(5):531-4.
The dependence of the activity of trypsin immobilized in polyacrylamide hydrogel on the hydrogel swelling ratio, the size distribution of its pores, and the means of enzyme binding has been studied. It has been shown that the most favorable conditions for immobilized trypsin are provided upon its binding to hydrogel via trypsin macromonomer copolymerization with acrylamide and a linking agent in the presence of a modifier that limits polymer chain growth.
研究了固定在聚丙烯酰胺水凝胶中的胰蛋白酶活性对水凝胶溶胀率、孔径分布和酶结合方式的依赖性。结果表明，当胰蛋白酶通过与丙烯酰胺和交联剂在限制聚合物链生长的改性剂存在下进行大分子单体共聚而与水凝胶结合时，可为固定化胰蛋白酶提供最有利的条件。
