测定磷脂酰胆碱氢过氧化物（PCOOH）作为膜脂质过氧化的标志物。
Determination of Phosphatidylcholine Hydroperoxide (PCOOH) as a Marker of Membrane Lipid Peroxidation.
作者信息
Nakagawa Kiyotaka, Kato Shunji, Miyazawa Teruo
机构信息
Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University.
出版信息
J Nutr Sci Vitaminol (Tokyo). 2015;61 Suppl:S78-80. doi: 10.3177/jnsv.61.S78.
Increasing evidence for phosphatidylcholine hydroperoxide (PCOOH) as a marker of oxidative food deterioration and oxidative diseases has revealed the need for a pure PCOOH standard and a reliable quantification method. Recently, we synthesized the PCOOH isomers 1-palmitoyl-2-(9-hydroperoxyoctadecadienoyl)-sn-glycero-3-phosphocholine, (16:0/9-HpODE PC) and 1-palmitoyl-2-(13-hydroperoxyoctadecadienoyl)-sn-glycero-3-phosphocholine (16:0/13-HpODE PC). Using these standards along with liquid chromatography-tandem mass spectrometry, a reliable quantification method was developed. This mini-review describes these analytical techniques, with a particular emphasis on clinical sample analysis.
越来越多的证据表明，磷脂酰胆碱氢过氧化物（PCOOH）可作为氧化食品变质和氧化疾病的标志物，这表明需要一种纯的PCOOH标准品和可靠的定量方法。最近，我们合成了PCOOH异构体1-棕榈酰-2-（9-氢过氧十八碳二烯酰基）-sn-甘油-3-磷酸胆碱（16:0/9-HpODE PC）和1-棕榈酰-2-（13-氢过氧十八碳二烯酰基）-sn-甘油-3-磷酸胆碱（16:0/13-HpODE PC）。使用这些标准品并结合液相色谱-串联质谱法，开发了一种可靠的定量方法。本综述介绍了这些分析技术，特别强调了临床样本分析。
Zotero 插件