Metabolomics Unit, College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee, United States of America.
Veterinary Diagnostic Laboratory, University of Kentucky, Lexington, Kentucky, United States of America.
PLoS One. 2018 Feb 23;13(2):e0193424. doi: 10.1371/journal.pone.0193424. eCollection 2018.
Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during infection have been described, but their utility as a diagnostic marker is still unclear. In this study, we undertook a lipidomics approach to determine if there are any differences in the serum lipid profiles of horses naturally infected with pathogenic Leptospira spp. and horses vaccinated against a commercially available bacterin. Utilizing a high-resolution mass spectrometry serum lipidomics analytical platform, we demonstrate that cyclic phosphatidic acids, diacylglycerols, and hydroperoxide oxidation products of choline plasmalogens are elevated in the serum of naturally infected as well as vaccinated horses. Other lipids of interest were triacylglycerols that were only elevated in the serum of infected horses and sphingomyelins that were increased only in the serum of vaccinated horses. This is the first report looking at the equine serum lipidome during leptospiral infection and vaccination.
目前用于钩端螺旋体病的诊断检测方法无法区分疫苗接种和感染血清抗体。已经描述了几种在感染过程中上调的钩端螺旋体蛋白，但它们作为诊断标志物的效用尚不清楚。在这项研究中，我们采用脂质组学方法来确定自然感染致病性钩端螺旋体的马和接种市售菌苗的马的血清脂质谱是否存在差异。利用高分辨率质谱血清脂质组学分析平台，我们证明环状磷脂酸、二酰基甘油和胆碱磷酯的过氧化物氧化产物在自然感染和接种疫苗的马的血清中升高。其他感兴趣的脂质是仅在感染马的血清中升高的三酰甘油和仅在接种疫苗的马的血清中升高的鞘氨醇。这是首次在钩端螺旋体感染和接种期间观察马的血清脂质组。
