Kato Shunji, Nakagawa Kiyotaka, Suzuki Yuuri, Suzuki Koichiro, Mizuochi Shunsuke, Miyazawa Teruo
Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University.
J Oleo Sci. 2014;63(5):431-7. doi: 10.5650/jos.ess13225. Epub 2014 Apr 9.
Increasing evidence of phospholipid peroxidation in the pathophysiology of various diseases demonstrates the need for pure phosphatidylcholine hydroperoxide (PCOOH) reference as a standard for quantification, and for biological studies in this field. Our previous study showed the usability of reaction between hydroperoxide and vinyl ether for preparation of PCOOH. However, the reaction has sometimes provided brown byproducts. Here, we report a method to improve reaction conditions, and demonstrate the production of 30 mg of PCOOH (a pure mixture of 13- and 9-hydroperoxyoctadecadienoyl PCOOH isomers) from 100 mg of phosphatidylcholine without byproduct formation. The resultant PCOOH will be useful as a standard for quantification studies, as well as for the evaluation of PCOOH pathogenicity.
越来越多的证据表明磷脂过氧化在各种疾病的病理生理学中存在，这表明需要纯的氢过氧化磷脂酰胆碱（PCOOH）作为定量标准以及该领域生物学研究的参考标准。我们之前的研究表明，氢过氧化物与乙烯基醚之间的反应可用于制备PCOOH。然而，该反应有时会产生棕色副产物。在此，我们报告一种改善反应条件的方法，并证明从100mg磷脂酰胆碱中可产生30mg PCOOH（13-和9-氢过氧化十八碳二烯酰PCOOH异构体的纯混合物）且无副产物形成。所得的PCOOH将作为定量研究的标准以及评估PCOOH致病性的有用工具。
