Singh Gurmeet K S, Balzer Ben W R, Kelly Patrick J, Paxton Karen, Hawke Catherine I, Handelsman David J, Steinbeck Katharine S
ANZAC Research Institute, University of Sydney, Camperdown, NSW, Australia.
Faculty of Pharmacy, Universiti Teknologi MARA, Selangor, Malaysia.
PLoS One. 2015 Nov 23;10(11):e0143555. doi: 10.1371/journal.pone.0143555. eCollection 2015.
BACKGROUND/AIMS: The longitudinal relationships of within-individual hormone and anthropometric changes during puberty have not ever been fully described. The objectives of this study were to demonstrate that 3 monthly urine collection was feasible in young adolescents and to utilise liquid chromatography-tandem mass spectrometry assay methods for serum and urine testosterone (T), estradiol (E2) and luteinizing hormone (LH) in adolescents by relating temporal changes in urine and serum hormones over 12 months to standard measures of pubertal development.
A community sample of 104 adolescents (57 female) was studied over 12 months with annual anthropometric assessment, blood sampling and self-rated Tanner staging and urine collected every 3 months. Serum and urine sex steroids (T, E2) were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and LH by immunoassay.
A high proportion (92%) of scheduled samples were obtained with low attrition rate of 6.7% over the 12 months. Urine hormone measurements correlated cross-sectionally and longitudinally with age, anthropometry and Tanner stage.
We have developed a feasible and valid sampling methodology and measurements for puberty hormones in urine, which allows a sampling frequency by which individual pubertal progression in adolescents can be described in depth.
背景/目的：青春期个体内激素与人体测量学变化之间的纵向关系尚未得到充分描述。本研究的目的是证明对于青少年而言，每3个月收集一次尿液是可行的，并通过将12个月内尿液和血清激素的时间变化与青春期发育的标准指标相关联，利用液相色谱 - 串联质谱分析法测定青少年血清和尿液中的睾酮（T）、雌二醇（E2）和促黄体生成素（LH）。
对104名青少年（57名女性）的社区样本进行了为期12个月的研究，每年进行人体测量评估、采血、自我评定坦纳分期，并每3个月收集一次尿液。血清和尿液中的性类固醇（T、E2）通过液相色谱 - 串联质谱法（LC-MS/MS）测定，LH通过免疫测定法测定。
在12个月内，高比例（92%）的预定样本被采集到，损耗率低至6.7%。尿液激素测量在横断面和纵向上均与年龄、人体测量学指标和坦纳分期相关。
我们已经开发出一种可行且有效的尿液青春期激素采样方法和测量方法，该方法允许以一定的采样频率深入描述青少年个体的青春期发育进程。
