Tan Hua-Wei, Song Xiao-Ming, Duan Wei-Ke, Wang Yan, Hou Xi-Lin
a State Key Laboratory of Crop Genetics and Germplasm Enhancement; Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
b Center of Genomics and Computational Biology, College of Life Sciences, North China University of Science and Technology, Tangshan 063000, China.
Genome. 2015 Nov;58(11):463-77. doi: 10.1139/gen-2015-0074.
The SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-box gene family contains highly conserved plant-specific transcription factors that play an important role in plant development, especially in flowering. Chinese cabbage (Brassica rapa subsp. pekinensis) is a leafy vegetable grown worldwide and is used as a model crop for research in genome duplication. The present study aimed to characterize the SBP-box transcription factor genes in Chinese cabbage. Twenty-nine SBP-box genes were identified in the Chinese cabbage genome and classified into six groups. We identified 23 orthologous and 5 co-orthologous SBP-box gene pairs between Chinese cabbage and Arabidopsis. An interaction network among these genes was constructed. Sixteen SBP-box genes were expressed more abundantly in flowers than in other tissues, suggesting their involvement in flowering. We show that the MiR156/157 family members may regulate the coding regions or 3'-UTR regions of Chinese cabbage SBP-box genes. As SBP-box genes were found to potentially participate in some plant development pathways, quantitative real-time PCR analysis was performed and showed that Chinese cabbage SBP-box genes were also sensitive to the exogenous hormones methyl jasmonic acid and salicylic acid. The SBP-box genes have undergone gene duplication and loss, evolving a more refined regulation for diverse stimulation in plant tissues. Our comprehensive genome-wide analysis provides insights into the SBP-box gene family of Chinese cabbage.
SQUAMOSA启动子结合蛋白（SBP）-盒基因家族包含高度保守的植物特异性转录因子，这些转录因子在植物发育尤其是开花过程中发挥重要作用。大白菜（Brassica rapa subsp. pekinensis）是一种在全球种植的叶菜类蔬菜，被用作基因组复制研究的模式作物。本研究旨在对大白菜中的SBP-盒转录因子基因进行表征。在大白菜基因组中鉴定出29个SBP-盒基因，并将其分为六组。我们在大白菜和拟南芥之间鉴定出23对直系同源和5对旁直系同源SBP-盒基因对。构建了这些基因之间的相互作用网络。16个SBP-盒基因在花中的表达量比在其他组织中更高，表明它们参与开花过程。我们发现MiR156/157家族成员可能调控大白菜SBP-盒基因的编码区或3'-UTR区。由于发现SBP-盒基因可能参与某些植物发育途径，因此进行了定量实时PCR分析，结果表明大白菜SBP-盒基因对外源激素茉莉酸甲酯和水杨酸也敏感。SBP-盒基因经历了基因复制和丢失，进化出了对植物组织中多种刺激更为精细的调控。我们全面的全基因组分析为大白菜SBP-盒基因家族提供了深入见解。
