β-羟基丁酸促进牛乳腺上皮细胞中由固醇调节元件结合蛋白1介导的脂肪酸合成。
β-Hydroxybutyrate Facilitates Fatty Acids Synthesis Mediated by Sterol Regulatory Element-Binding Protein1 in Bovine Mammary Epithelial Cells.
作者信息
Zhang Min, Zhang Shiqi, Hui Qi, Lei Lin, Du Xiliang, Gao Wenwen, Zhang Renhe, Liu Guowen, Li Xiaobing, Li Xinwei
出版信息
Cell Physiol Biochem. 2015;37(6):2115-24. doi: 10.1159/000438569. Epub 2015 Nov 25.
BACKGROUND/AIMS: In dairy cows, β-hydroxybutyrate (BHBA) is utilized as precursors of de novo synthesized fatty acids in mammary gland. Ketotic cows are characterized by excessive negative energy balance (NEB), which can further increase the blood BHBA concentration. Sterol regulatory element-binding protein1 (SREBP1) and cell death-inducing DNA fragmentation factor-alpha-like effector α (Cidea) play crucial roles in lipid synthesis. Therefore, we hypothesized that BHBA could stimulate SREBP1/Cidea pathway to increase milk fat synthesis in bovine mammary epithelial cells.
METHODS
Bovine mammary epithelial cells were treated with different concentrations of BHBA and transfected with adenovirus to silence SREBP1 expression. The effects of BHBA on the lipid synthesis in bovine mammary epithelial cells were investigated.
RESULTS
The results showed that BHBA could significantly increase the expression of SREBP1, fatty acid synthase (FAS), acetyl-CoA carboxylase α (ACC-α), Cidea and diacylglycerol transferase-1 (DGAT-1), as well as the triglycerides (TG) content in bovine mammary epithelial cells. BHBA treatment also increased the transfer of mature SREBP1 to nucleus compared with control group. However, SREBP1 silencing could significantly down-regulate the overexpression of FAS, ACC-α, Cidea and DGAT-1, as well as TG content induced by BHBA.
CONCLUSION
The present data indicate that BHBA can significantly increase TG secretion mediated by SREBP1 in bovine mammary epithelial cells.
背景/目的：在奶牛中，β-羟基丁酸（BHBA）被用作乳腺中从头合成脂肪酸的前体。酮病奶牛的特征是能量负平衡（NEB）过度，这会进一步增加血液中BHBA的浓度。固醇调节元件结合蛋白1（SREBP1）和细胞死亡诱导DNA片段化因子α样效应器α（Cidea）在脂质合成中起关键作用。因此，我们假设BHBA可以刺激SREBP1/Cidea途径以增加牛乳腺上皮细胞中的乳脂肪合成。
方法
用不同浓度的BHBA处理牛乳腺上皮细胞，并用腺病毒转染以沉默SREBP1表达。研究了BHBA对牛乳腺上皮细胞脂质合成的影响。
结果
结果表明，BHBA可显著增加牛乳腺上皮细胞中SREBP1、脂肪酸合酶（FAS）、乙酰辅酶A羧化酶α（ACC-α）、Cidea和二酰甘油转移酶-1（DGAT-1）的表达，以及甘油三酯（TG）含量。与对照组相比，BHBA处理还增加了成熟SREBP1向细胞核的转移。然而，沉默SREBP1可显著下调BHBA诱导的FAS、ACC-α、Cidea和DGAT-1的过表达以及TG含量。
结论
目前的数据表明，BHBA可显著增加牛乳腺上皮细胞中由SREBP1介导的TG分泌。
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