Yin G R, Wang Q, Zhang X B, Wang S J
Department of Joint Surgery, The Second Hospital of Shandong University, Jinan, China.
DeZhou University, DeZhou, China.
Genet Mol Res. 2015 Nov 13;14(4):14246-52. doi: 10.4238/2015.November.13.8.
Osteosarcoma is a highly malignant cancer that often appears in teenagers. It is the most frequently occurring primary bone tumor, and can easily metastasize, resulting in high mortality. MicroRNAs express abnormally in osteosarcoma, and may function as oncogenes or tumor suppressors. Recent studies showed that microRNA184 (miR-184) is abnormally expressed in multiple tumors, and is involved in tumor cell growth, differentiation, invasion, and metastasis. Nevertheless, the role of miR-184 in osteosarcoma cells remains unknown. We evaluated the expression and function of microRNA184 in osteosarcoma cells. SOSP-M osteosarcoma cells were divided into normal control, miR-184 mimic, and miR-184 inhibitor groups. Real-time PCR was applied to detect miR-184 expression. The 3-(4,5-dimethylthaizol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to evaluate cell proliferation. Transwell assays were performed to detect changes in cell invasion ability. Compared with the control group, miR-184 expression was significantly increased in the miR-184 mimic group (P < 0.05). After miR-184 inhibitor transfection, miR-184 expression was obviously reduced (P < 0.05). Tumor cell proliferation was enhanced in the miR-184 mimic group (P < 0.05), whereas miR-184 inhibition suppressed cell proliferation (P < 0.05). Furthermore, tumor cell invasion increased after miR-184 mimic transfection (P < 0.05), and decreased after inhibiting miR-184 (P < 0.05). MiR-184 promotes tumor cell proliferation and invasion, and may represent a new biological target for osteosarcoma.
骨肉瘤是一种常出现在青少年中的高度恶性癌症。它是最常见的原发性骨肿瘤，且容易转移，导致高死亡率。微小RNA在骨肉瘤中表达异常，可能发挥癌基因或肿瘤抑制基因的作用。最近的研究表明，微小RNA184（miR - 184）在多种肿瘤中表达异常，并参与肿瘤细胞的生长、分化、侵袭和转移。然而，miR - 184在骨肉瘤细胞中的作用仍不清楚。我们评估了微小RNA184在骨肉瘤细胞中的表达和功能。将SOSP - M骨肉瘤细胞分为正常对照组、miR - 184模拟物组和miR - 184抑制剂组。应用实时定量聚合酶链反应检测miR - 184的表达。采用3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐法评估细胞增殖。进行Transwell实验检测细胞侵袭能力的变化。与对照组相比，miR - 184模拟物组中miR - 184表达显著增加（P < 0.05）。转染miR - 184抑制剂后，miR - 184表达明显降低（P < 0.05）。miR - 184模拟物组中肿瘤细胞增殖增强（P < 0.05），而抑制miR - 184则抑制细胞增殖（P < 0.05）。此外，转染miR - 184模拟物后肿瘤细胞侵袭增加（P < 0.05），抑制miR - 184后侵袭减少（P < 0.05）。MiR - 184促进肿瘤细胞增殖和侵袭，可能是骨肉瘤的一个新的生物学靶点。
