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人毛细血管扩张性骨肉瘤癌细胞系中微小RNA表达特征的初步分析

Analysis of a Preliminary microRNA Expression Signature in a Human Telangiectatic Osteogenic Sarcoma Cancer Cell Line.

作者信息

Palmini Gaia, Romagnoli Cecilia, Donati Simone, Zonefrati Roberto, Galli Gianna, Marini Francesca, Iantomasi Teresa, Aldinucci Alessandra, Leoncini Gigliola, Franchi Alessandro, Beltrami Giovanni, Campanacci Domenico Andrea, Capanna Rodolfo, Brandi Maria Luisa

机构信息

Department of Experimental and Clinical Biomedical Sciences, University of Florence, 50134 Florence, Italy.

Central Laboratory, Azienda Ospedaliero-Universitaria Careggi, 50134 Florence, Italy.

出版信息

Int J Mol Sci. 2021 Jan 25;22(3):1163. doi: 10.3390/ijms22031163.

DOI:10.3390/ijms22031163
PMID:33503899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7866083/
Abstract

Telangiectatic osteosarcoma (TOS) is an aggressive variant of osteosarcoma (OS) with distinctive radiographic, gross, microscopic features, and prognostic implications. Despite several studies on OS, we are still far from understanding the molecular mechanisms of TOS. In recent years, many studies have demonstrated not only that microRNAs (miRNAs) are involved in OS tumorigenesis, development, and metastasis, but also that the presence in high-grade types of OS of cancer stem cells (CSCs) plays an important role in tumor progression. Despite these findings, nothing has been described previously about the expression of miRNAs and the presence of CSCs in human TOS. Therefore, we have isolated/characterized a putative CSC cell line from human TOS (TOS-CSCs) and evaluated the expression levels of several miRNAs in TOS-CSCs using real-time quantitative assays. We show, for the first time, the existence of CSCs in human TOS, highlighting the in vitro establishment of this unique stabilized cell line and an identification of a preliminary expression of the miRNA profile, characteristic of TOS-CSCs. These findings represent an important step in the study of the biology of one of the most aggressive variants of OS and the role of miRNAs in TOS-CSC behavior.

摘要

毛细血管扩张性骨肉瘤（TOS）是骨肉瘤（OS）的一种侵袭性变体，具有独特的影像学、大体、显微镜特征及预后意义。尽管对骨肉瘤已有多项研究，但我们对毛细血管扩张性骨肉瘤的分子机制仍知之甚少。近年来，许多研究表明，微小RNA（miRNA）不仅参与骨肉瘤的肿瘤发生、发展和转移，而且高级别骨肉瘤中癌症干细胞（CSC）的存在在肿瘤进展中起重要作用。尽管有这些发现，但此前关于人类毛细血管扩张性骨肉瘤中miRNA的表达及癌症干细胞的存在尚未有描述。因此，我们从人类毛细血管扩张性骨肉瘤中分离/鉴定出一种假定的癌症干细胞系（TOS-CSCs），并使用实时定量分析法评估了TOS-CSCs中几种miRNA的表达水平。我们首次证明了人类毛细血管扩张性骨肉瘤中癌症干细胞的存在，突出了这种独特稳定细胞系的体外建立以及对TOS-CSCs特有的miRNA谱初步表达的鉴定。这些发现代表了对骨肉瘤最具侵袭性变体之一的生物学研究以及miRNA在TOS-CSC行为中的作用研究的重要一步。

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