Wang Lei, Zhang Mei, Liu Dong-Xu
School of Dentistry, Shandong University Jinan, Shandong Province, China ; Department of Stomatology, Qilu Hospital of Shandong University Jinan, Shandong Province, China.
Department of Dental, Shandong Medical College Jinan, Shandong Province, China.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):5495-504. eCollection 2014.
This study aims to explore the clinical characteristics of ATP binding cassette E1 (ABCE1) in oral squamous cell carcinomas (OSCC) and its roles in the proliferation, invasiveness, migration and apoptosis of the human oral squamous cell carcinoma cells CAL-27.
The expression of ABCE1 and its target protein-RNase L, were first studied in tumor tissues of OSCC and adjacent non-tumor tissues. Moreover, CAL-27cells were transfected by ABCE1-specific shRNA, then MTT assay, the transwell and scratch assay were used to study cell proliferation and migration activity; the apoptosis rate and cell cycle distribution were tested by flow cytometry. Western blot and RT-PCR assay were adopted to measure their silencing efficacy.
ABCE1 expression is low in the adjacent non-tumor tissues while the expression is high in the oral cancer; the expression is reversely proportional to the differentiation degrees. The expression of RNaseL was in contrary to ABCE1. After transfected with ABCE1-siRNA, the proliferation, invasiveness and migration capabilities of cells decreased significantly whilst the apoptosis rate enhanced greatly (P < 0.01). Meanwhile, the expression of ABCE1 in CAL-27 cells was blocked (P < 0.01) while the expression of RNase L increased significantly (P < 0.01).
ABCE1 is closely connected with the pathogenesis and development of oral cancer, which acts through the cellular pathways of 2-5A/RNase L.
本研究旨在探讨三磷酸腺苷结合盒转运体E1（ABCE1）在口腔鳞状细胞癌（OSCC）中的临床特征及其在人口腔鳞状癌细胞CAL-27增殖、侵袭、迁移和凋亡中的作用。
首先研究ABCE1及其靶蛋白核糖核酸酶L（RNase L）在OSCC肿瘤组织及癌旁非肿瘤组织中的表达。此外，用ABCE1特异性短发夹RNA（shRNA）转染CAL-27细胞，然后采用MTT法以及Transwell和划痕试验研究细胞增殖和迁移活性；通过流式细胞术检测细胞凋亡率和细胞周期分布。采用蛋白质免疫印迹法（Western blot）和逆转录-聚合酶链反应（RT-PCR）检测其沉默效果。
ABCE1在癌旁非肿瘤组织中表达较低，而在口腔癌中表达较高；其表达与分化程度呈负相关。RNase L的表达与ABCE1相反。转染ABCE1小干扰RNA（siRNA）后，细胞的增殖、侵袭和迁移能力显著降低，而凋亡率显著提高（P<0.01）。同时，CAL-27细胞中ABCE1的表达被阻断（P<0.01），而RNase L的表达显著增加（P<0.01）。
ABCE1与口腔癌的发病机制和发展密切相关，其通过2-5A/RNase L细胞途径发挥作用。
