Ribas-Silva R C, Ribas A D, Ferreira E C, Silveira T G V, Borelli S D
Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, PR, Brasil
Programa de Pós-Graduação em Ciências da Saúde, Universidade Estadual de Maringá, Maringá, PR, Brasil.
Genet Mol Res. 2015 Nov 23;14(4):14929-35. doi: 10.4238/2015.November.18.58.
Leishmaniasis is a parasitic infectious disease with global repercussions. American cutaneous leishmaniasis (ACL) is endemic in southern Brazil and its pathogenesis varies according to parasite species, immune response, and host genetics. In terms of immunogenetics, many host genes, including HLA (human leukocyte antigen), could be involved in susceptibility to and protection against ACL. Accordingly, the aim of this study was to investigate the association between HLA class I genes (HLA-A, -B, and -C) and ACL in an endemic region of southern Brazil. The allele frequencies of 186 patients diagnosed with ACL and 278 healthy individuals were compared. HLA class I (HLA-A, -B, and -C) typing was carried out by PCR-SSO using Luminex technology. The results revealed an association between the HLA-C04 allele and the patient study group, in which it appeared more frequently than in the control group [21.5 vs 13.49% (P = 0.0016 and Pc = 0.0258; OR = 1.7560; 95%CI = 1.2227-2.5240)], thereby suggesting an increased susceptibility to ACL. Additional allelic groups such as HLA-A02, HLA-B35, HLA-B45, HLA-C01, and HLA-C15 were also implicated; however, further investigation is necessary to confirm their association with ACL. Therefore, the results obtained in this study demonstrate the involvement of HLA class I genes in the susceptibility or resistance to ACL, with significant association between HLA-C*04 and ACL susceptibility.
利什曼病是一种具有全球影响的寄生虫传染病。美洲皮肤利什曼病（ACL）在巴西南部流行，其发病机制因寄生虫种类、免疫反应和宿主基因而异。在免疫遗传学方面，许多宿主基因，包括人类白细胞抗原（HLA），可能与ACL的易感性和抵抗力有关。因此，本研究的目的是调查巴西南部一个流行地区HLA I类基因（HLA - A、- B和- C）与ACL之间的关联。比较了186例被诊断为ACL的患者和278例健康个体的等位基因频率。使用Luminex技术通过PCR - SSO进行HLA I类（HLA - A、- B和- C）分型。结果显示HLA - C04等位基因与患者研究组之间存在关联，其在患者组中的出现频率高于对照组[21.5%对13.49%（P = 0.0016，Pc = 0.0258；OR = 1.7560；95%CI = 1.2227 - 2.5240）]，从而表明对ACL的易感性增加。其他等位基因组，如HLA - A02、HLA - B35、HLA - B45、HLA - C01和HLA - C15也有涉及；然而，需要进一步研究以证实它们与ACL的关联。因此，本研究获得的结果表明HLA I类基因参与了对ACL的易感性或抵抗力，HLA - C*04与ACL易感性之间存在显著关联。
