Shi Fan, He Hao, Wang Yintao, Liu Dayong, Hu Minglie, Wang Chingyue
Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, 300072, China.
Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, 300072, China ; Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Biomed Opt Express. 2015 Oct 23;6(11):4539-45. doi: 10.1364/BOE.6.004539. eCollection 2015 Nov 1.
Mitochondria play a key role in all cellular physiology, processes, and behaviors. It is very difficult to precisely stimulate single mitochondria noninvasively in traditional biomedical research. In this study, we report that femtosecond laser can stimulate fragmentation or swelling of single mitochondria in human mesenchymal stem cells rather than physical disruption or ablation. In experiments, fragmented mitochondria can recover normal very soon but swelling ones cannot. At the same time, laser-induced generation of mitochondrial reactive oxygen species and opening of mitochondria permeability transition pores are involved in mitochondrial responses to photostimulation. Furthermore, the localized translocation of proapoptotic molecules are found in those stimulated mitochondria. Those results suggest femtosecond-laser photostimulation as a noninvasive and precise method for mitochondrial manipulation and related research.
线粒体在所有细胞生理、过程及行为中发挥着关键作用。在传统生物医学研究中，很难对单个线粒体进行精确的非侵入性刺激。在本研究中，我们报告飞秒激光可刺激人骨髓间充质干细胞中单个线粒体发生碎片化或肿胀，而非物理性破坏或消融。在实验中，碎片化的线粒体很快就能恢复正常，而肿胀的线粒体则不能。同时，激光诱导产生的线粒体活性氧以及线粒体通透性转换孔的开放参与了线粒体对光刺激的反应。此外，在那些受到刺激的线粒体中发现了促凋亡分子的局部易位。这些结果表明飞秒激光光刺激是一种用于线粒体操作及相关研究的非侵入性精确方法。
